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Literature summary for 2.7.4.1 extracted from

  • Ishige, K.; Zhang, H.; Kornberg, A.
    Polyphosphate kinase (PPK2), a potent, polyphosphate-driven generator of GTP (2002), Proc. Natl. Acad. Sci. USA, 99, 16684-16688.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 GDP
-
 Pseudomonas aeruginosa
0.75
-
 ADP
-
 Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5fold higher activation than Mn2+, at optimal concentration of 10 mM Pseudomonas aeruginosa
Mn2+ Mg2+ shows 5fold higher activation than Mn2+, at optimal concentration of 10 mM Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 4 * 41000 Pseudomonas aeruginosa
41000
-
 8 * 41000, in presence of P15, octamer is unstable Pseudomonas aeruginosa
164000
-
 tetramer, gel filtration Pseudomonas aeruginosa
358000
-
 unstable octamer, gel filtration Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 PAOM5
-
Pseudomonas aeruginosa PAOM5
-
 PAOM5
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (phosphate)n+1 GDP is preferred over ADP over nucleoside diphosphate acceptors Pseudomonas aeruginosa ATP + (phosphate)n
-
 ?
ADP + (phosphate)n+1 GDP is preferred over ADP over nucleoside diphosphate acceptors Pseudomonas aeruginosa PAOM5 ATP + (phosphate)n
-
 ?
GDP + (phosphate)n+1 chains of 30-50 residues are optimal, chains of 15-700 residues can also serve. GDP is preferred over ADP over nucleoside diphosphate acceptors Pseudomonas aeruginosa GTP + (phosphate)n
-
 ?
GDP + (phosphate)n+1 chains of 30-50 residues are optimal, chains of 15-700 residues can also serve. GDP is preferred over ADP over nucleoside diphosphate acceptors Pseudomonas aeruginosa PAOM5 GTP + (phosphate)n
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 41000, in presence of P15, octamer is unstable Pseudomonas aeruginosa
tetramer 4 * 41000 Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
PPK2
-
 Pseudomonas aeruginosa