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Literature summary for 2.7.4.1 extracted from

  • Zhang, H.; Ishige, K.; Kornberg, A.
    A polyphosphate kinase (PPK2) widely conserved in bacteria (2002), Proc. Natl. Acad. Sci. USA, 99, 16678-16683.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.1

Activating Compound

Activating Compound Comment Organism Structure
Polyphosphate activation of polyphosphate kinase 2 Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
expression of polyphosphate kinase 2 gene in Escherichia coli Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 ADP pH 7.2, 37°C Pseudomonas aeruginosa
0.68
-
 GDP pH 7.2, 37°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Pseudomonas aeruginosa
Mg2+ polyphosphate kinase 2, preferred in ATP synthesis over Mn2+ Pseudomonas aeruginosa
Mn2+ polyphosphate kinase 2, 10 mM preferred in polyphosphate synthesis over Mg2+ Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40800
-
 x * 40800, deduced from nucleotide sequence Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa Q8GCQ3 polyphosphate kinase 2
-

Purification (Commentary)

Purification (Comment) Organism
heparin-Sepharose, GTP agarose, Mono Q, Mono S Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0055
-
 polyphosphate kinase 2 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (phosphate)n polyphosphate kinase 1 and 2 Pseudomonas aeruginosa ADP + (phosphate)n+1 polyphosphate with 200-800 residues ?
ATP + (phosphate)n polyphosphate kinase 1 and 2 Pseudomonas aeruginosa ADP + (phosphate)n+1 poyphosphate with 500-800 residues ?
GTP + (phosphate)n polyphosphate kinase 2 Pseudomonas aeruginosa GDP + (phosphate)n+1
-
 ?

Subunits

Subunits Comment Organism
? x * 40800, deduced from nucleotide sequence Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
PPK2 i.e. polyphosphate kinase 2 Pseudomonas aeruginosa