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Literature summary for 2.7.4.1 extracted from

  • Levinson, S.L.; Jacobs, L.H.; Krulwich, T.A.; Li, H.C.
    Purification and characterization of a polyphosphate kinase from Arthrobacter atrocyaneus (1975), J. Gen. Microbiol., 88, 65-74.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
basic protein activation Sinomonas atrocyanea
basic protein can substitute for histone Sinomonas atrocyanea
bovine serum albumin activation, can substitute for histone only in the absence of phosphate Sinomonas atrocyanea
casein activation, can substitute for histone only in the absence of phosphate Sinomonas atrocyanea
histone required for activity Sinomonas atrocyanea
phosphate
-
Sinomonas atrocyanea

Inhibitors

Inhibitors Comment Organism Structure
ADP 4 mM, product inhibition Sinomonas atrocyanea
ATP free form, above 0.008 mM Sinomonas atrocyanea
diphosphate 1 mM, complete inhibition Sinomonas atrocyanea
F- 20 mM, complete inhibition Sinomonas atrocyanea
Mg2+ free form, above 6 mM, activating below Sinomonas atrocyanea
Mn2+ free form, above 2 mM, activating below Sinomonas atrocyanea
MnATP2- at high concentrations, above 0.6 mM, activating below Sinomonas atrocyanea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.53
-
ATP pH 7.0, 30°C Sinomonas atrocyanea
0.53
-
ATP ATP in form of MnATP2- Sinomonas atrocyanea
1.67
-
phosphate pH 7.0, 30°C Sinomonas atrocyanea

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Sinomonas atrocyanea
Mg2+ inhibitory above 6 mM Sinomonas atrocyanea
Mg2+ active substrate: MgATP Sinomonas atrocyanea
Mn2+ activation, can replace Mg2+ Sinomonas atrocyanea
Mn2+ maximal activity at 1.0-2.0 mM, inhibition above 2 mM Sinomonas atrocyanea
Mn2+ active substrate: MnATP Sinomonas atrocyanea

Organism

Organism UniProt Comment Textmining
Sinomonas atrocyanea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, Sephadex G-200 Sinomonas atrocyanea

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.82
-
-
Sinomonas atrocyanea

Storage Stability

Storage Stability Organism
5°C, at least 6 months Sinomonas atrocyanea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (phosphate)n incorporates gamma-phosphate of ATP into long-chain polyphosphate molecules Sinomonas atrocyanea ADP + (phosphate)n+1
-
?
ATP + phosphate
-
Sinomonas atrocyanea ADP + diphosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Sinomonas atrocyanea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
Sinomonas atrocyanea

pH Range

pH Minimum pH Maximum Comment Organism
5 8 approx. half-maximal activity at pH 5.0 and pH 8.0 Sinomonas atrocyanea