Cloned (Comment) | Organism |
---|---|
the C-terminal and N-terminal domains of enzyme I are expressed in Escherichia coli BL21star(DE3) cells | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
calculated from amino acid sequence | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the C-terminal domain can phosphorylate the N-terminal domain of enzyme I, but their binding is transient regardless of the presence of phosphoenolpyruvate | Escherichia coli |
Purification (Comment) | Organism |
---|---|
DEAE column chromatography and Superdex 200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + protein histidine | enzyme I catalyzes two reversible reactions: an Mg2+-dependent autophosphorylation reaction using phosphoenolpyruvate as a substrate, and a phosphotransfer reaction to histidine-containing phosphocarrier protein | Escherichia coli | pyruvate + protein Npi-phospho-L-histidine | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
EIC | C-terminal domain of enzyme I | Escherichia coli |
EIN | N-terminal domain of enzyme I | Escherichia coli |