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Literature summary for 2.7.3.9 extracted from
- Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties (1980), Can. J. Biochem., 58, 40-48.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory: AMP, ADP, ATP, GMP, GDP, GTP, cAMP, cGMP, acetyl-CoA, acetyl phosphate, glucose 6-phosphate, glucose 1-phosphate, fructose 1,6-diphosphate, L-histidine, D-histidine | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0105 | - |
histidine-containing protein | 23°C, pH6.5 | Escherichia coli | |
| 0.18 | - |
phosphoenolpyruvate | 23°C, pH6.5 | Escherichia coli |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Escherichia coli | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
2 * 65000, SDS-PAGE, gel filtration at 4-6°C | Escherichia coli |
| 135000 | - |
gel filtration at room temperature, sedimentation velocity | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| phosphoenolpyruvate + protein histidine = pyruvate + protein Npi-phospho-L-histidine | bi-bi ping-pong mechanism | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + histidine-containing protein | - |
Escherichia coli | pyruvate + phosphorylated histidine-containing protein | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | the enzyme is dimeric at room temperature and dissociates to a catalytically not active monomer at 4-6°C | Escherichia coli |
| dimer | 2 * 65000, SDS-PAGE, gel filtration at 4-6°C | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 8.5 | less than 50% of maximal activity above and below | Escherichia coli |
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Release 2023.1 (January 2023)
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