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Literature summary for 2.7.3.7 extracted from

  • van Thoai, N.; di Jeso, F.; Robin, Y.; der Terrossian, E.
    Sur la nouvelle acide adenosine 5'-triphosphorique:guanidine phosphotransferase, l‘opheline kinase (1966), Biochim. Biophys. Acta, 113, 542-550.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Chloroacetophenone
-
Ophelia neglecta
monoiodoacetate
-
Ophelia neglecta
NEM
-
Ophelia neglecta
PCMB
-
Ophelia neglecta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.85
-
ADP 30°C Ophelia neglecta
1.1
-
phosphoopheline 30°C Ophelia neglecta
1.8
-
phosphotaurocyamine 30°C Ophelia neglecta
3
-
ATP 30°C Ophelia neglecta
5.8
-
opheline 30°C Ophelia neglecta
13
-
2-guanidinoethyl phosphate 30°C Ophelia neglecta
15
-
lombricine 30°C Ophelia neglecta
50
-
Taurocyamine 30°C Ophelia neglecta

Organism

Organism UniProt Comment Textmining
Ophelia neglecta
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Ophelia neglecta

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Ophelia neglecta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-guanidinoethyl phosphate
-
Ophelia neglecta ADP + N-phospho-2-guanidinoethyl phosphate
-
?
ATP + lombricine
-
Ophelia neglecta ADP + phospholombricine
-
?
ATP + opheline i.e. 2-guanidinoethylmethyl phosphate Ophelia neglecta ADP + phosphoopheline
-
r
ATP + taurocyamine
-
Ophelia neglecta ADP + phosphotaurocyamine
-
r
additional information no phosphorylation of arginine, guanidinoacetate, no reverse reaction with their corresponding phosphagens Ophelia neglecta ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
Ophelia neglecta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
reaction with phosphoopheline Ophelia neglecta
8.5
-
phosphorylation of opheline Ophelia neglecta

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 oH 6.0: about 80% of maximal activity, pH 7.5: about 65% of maximal activity, reaction with phosphoopheline Ophelia neglecta
7.5 9 pH 7.5: about 65% of maximal activity, pH 9.0: about 50% of maximal activity, phosphorylation of opheline Ophelia neglecta