Literature summary for 2.7.3.4 extracted from

Merceron, R.; Awama, A.M.; Montserret, R.; Marcillat, O.; Gouet, P.
The substrate-free and -bound crystal structures of the duplicated taurocyamine kinase from the human parasite Schistosoma mansoni (2015), J. Biol. Chem., 290, 12951-12963.

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha	Schistosoma mansoni

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type and mutant enzymes complexed with taurocyamine or L-arginine, sitting drop vapor diffusion method, mixing of 5 mg/ml protein in 20mM Tris-HCl, pH 8.5, with reservoir solution, containing 200mM diammonium tartrate, pH 5.4, 20% w/v PEG 3350, 20% v/v ethylene glycol, in a 2:1 or 1:1 protein/solution volume ratio, 2 weeks, 20°C, X-ray diffraction structure determination and analysis at 2.2 A resolution leads to a small angle x-ray scattering model of SmTK-TSA in solution with two closed active sites, molecular replacement. The SmTK crystal is soaked with the dead end transition state analogue components taurocyamine-NO3 2-MgADP	Schistosoma mansoni

Crystallization (Comment)

Organism

purified recombinant wild-type and mutant enzymes complexed with taurocyamine or L-arginine, sitting drop vapor diffusion method, mixing of 5 mg/ml protein in 20mM Tris-HCl, pH 8.5, with reservoir solution, containing 200mM diammonium tartrate, pH 5.4, 20% w/v PEG 3350, 20% v/v ethylene glycol, in a 2:1 or 1:1 protein/solution volume ratio, 2 weeks, 20°C, X-ray diffraction structure determination and analysis at 2.2 A resolution leads to a small angle x-ray scattering model of SmTK-TSA in solution with two closed active sites, molecular replacement. The SmTK crystal is soaked with the dead end transition state analogue components taurocyamine-NO3 2-MgADP

Schistosoma mansoni

Protein Variants

Protein Variants	Comment	Organism
C268S	site-directed mutagenesis	Schistosoma mansoni
C268S/C631S	site-directed mutagenesis	Schistosoma mansoni
C631S	site-directed mutagenesis	Schistosoma mansoni

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on	Schistosoma mansoni

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + taurocyamine	Schistosoma mansoni	-	ADP + N-phosphotaurocyamine	-	r

Organism

Organism	UniProt	Comment	Textmining
Schistosoma mansoni	P16641	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by Blue Sepharose affinity and anion exchange chromatography, followed by ultrafiltration	Schistosoma mansoni

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + taurocyamine = ADP + N-phosphotaurocyamine	an arginine residue of the phosphagen specificity loop is crucial for substrate specificity, mechanism and structure-function relationship, overview	Schistosoma mansoni	a

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + taurocyamine	-	Schistosoma mansoni	ADP + N-phosphotaurocyamine	-	r
ATP + taurocyamine	the enzyme catalyzes the reversibleMg2-dependent transfer of a phosphoryl group between ATP and taurocyamine	Schistosoma mansoni	ADP + N-phosphotaurocyamine	-	r

Subunits

Subunits	Comment	Organism
More	the two unliganded lobes present a canonical open conformation and interact via their respective C- and N-terminal domains at a helix-mediated interface. The two lobes function independently	Schistosoma mansoni

Cofactor

Cofactor	Comment	Organism	Structure
ADP	-	Schistosoma mansoni
ATP	-	Schistosoma mansoni

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the phosphagen kinase (PK) family. Schistosoma mansoni taurocyamine SmTK is derived from gene duplication, as are all known trematode taurocyamine kinases	Schistosoma mansoni