Application | Comment | Organism |
---|---|---|
drug development | Schistosoma mansoni is a trematode flatworm that has been identified as the aetiological agent of schistosomiasis, a disease that affects about 200 million people worldwide | Schistosoma mansoni |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapour-diffusion, 100 mM MES, pH 6.5, 25%(w/v) PEG 3000, 290 K, unit-cell parameters a = 52.7, b = 122.1, c = 63.2 A , beta = 108.5°, space group P21, 2.8 A resolution on ESRF beamline ID29 | Schistosoma mansoni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
- |
Schistosoma mansoni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schistosoma mansoni | P16641 | cercariae, Puerto Rican strain, cloned into a pET21 plasmid without any tag, expression in Escherichia coli BL21 (DE3) | - |
Purification (Comment) | Organism |
---|---|
affinity chromatogrphy, concentrated to 10 mg ml-1 by lyophilization and resolubilized in MilliQ water, the purity is verified by the presence of a single band on overloaded Coomassie Blue-stained SDS-PAGE | Schistosoma mansoni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | recombinant enzyme shows catalytic activity with several guanidylic compounds, the highest activity being measured with taurocyamine, taurocyamine may not be the physiological substrate and that Schistosoma mansoni may use another phosphagen | Schistosoma mansoni | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
guanidino kinase | - |
Schistosoma mansoni |
SmGK | - |
Schistosoma mansoni |