Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.3.4 extracted from

  • Awama, A.M.; Paracuellos, P.; Laurent, S.; Dissous, C.; Marcillat, O.; Gouet, P.
    Crystallization and x-ray analysis of the Schistosoma mansoni guanidino kinase (2008), Acta Crystallogr. Sect. F, F64, 854-857.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development Schistosoma mansoni is a trematode flatworm that has been identified as the aetiological agent of schistosomiasis, a disease that affects about 200 million people worldwide Schistosoma mansoni

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour-diffusion, 100 mM MES, pH 6.5, 25%(w/v) PEG 3000, 290 K, unit-cell parameters a = 52.7, b = 122.1, c = 63.2 A , beta = 108.5°, space group P21, 2.8 A resolution on ESRF beamline ID29 Schistosoma mansoni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
-
Schistosoma mansoni

Organism

Organism UniProt Comment Textmining
Schistosoma mansoni P16641 cercariae, Puerto Rican strain, cloned into a pET21 plasmid without any tag, expression in Escherichia coli BL21 (DE3)
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatogrphy, concentrated to 10 mg ml-1 by lyophilization and resolubilized in MilliQ water, the purity is verified by the presence of a single band on overloaded Coomassie Blue-stained SDS-PAGE Schistosoma mansoni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information recombinant enzyme shows catalytic activity with several guanidylic compounds, the highest activity being measured with taurocyamine, taurocyamine may not be the physiological substrate and that Schistosoma mansoni may use another phosphagen Schistosoma mansoni ?
-
?

Synonyms

Synonyms Comment Organism
guanidino kinase
-
Schistosoma mansoni
SmGK
-
Schistosoma mansoni