Cloned (Comment) | Organism |
---|---|
gene AK1 or Tb927.9.6170, located on chromosome 9, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression of full-length and truncated versions of epitope tagged isozyme AK1 | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a stable isozyme AK1 knockout DELTAak1 line | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
flagellum | isozyme AK1, the 22 N-terminal amino acids are responsible for flagellum targeting. The flagellar targeting sequence of AK1 shares close similarity with that of calflagins. The flagellar localisation of AK1 persists throughout the Trypanosoma brucei life cycle, overview | Trypanosoma brucei | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | Q9U420 | gene AK1 = gene Tb927.9.6170 | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
AK1 | - |
Trypanosoma brucei |
Tb927.9.6170 | - |
Trypanosoma brucei |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trypanosoma brucei | sequence calculation | - |
4.9 |
General Information | Comment | Organism |
---|---|---|
evolution | arginine kinase genes in trypanosomatids, phylogenetic analysis, overview | Trypanosoma brucei |
malfunction | RNAi knockdown of all three arginine kinase isozymes induces a growth defect that is more prominent when the cells are exposed to oxidative stress. Loss of flagellar isozyme AK1 reduces swim velocity without visible alteration of flagellar morphology. The absence of isozyme AK1 results in reduced infectivity by procyclic trypanosomes for tsetse flies | Trypanosoma brucei |
additional information | isozyme AK1 has two insertions of respectively 22 and 26 amino acids at the N- and C-terminus | Trypanosoma brucei |
physiological function | isozyme AK1 confers a competitive advantage in infections of tsetse flies in midgut by the parasite, overview | Trypanosoma brucei |