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Literature summary for 2.7.3.3 extracted from
- Cloning, expression, purification, and characterization of arginine kinase from Locusta migratoria manilensis (2007), Comp. Biochem. Physiol. B, 148B, 355-362.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli by two prokaryotic expression plasmids, pET-30a and pET-28a28a. The recombinant protein is expressed as inclusion bodies using pET-30a. Using expression plasmid, pET-28a, and changing the expression conditions results in a soluble and functional form of arginine kinase | Locusta migratoria manilensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.951 | - |
L-arginine | - |
Locusta migratoria manilensis |  |
| 1.27 | - |
ATP | - |
Locusta migratoria manilensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Locusta migratoria manilensis | A6M9J4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Locusta migratoria manilensis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| after denaturation, the recombinant AK is successfully renatured and confirmed to be enzymatically active. Addition of Tween-20 and SDS to the dilution system lead to higher renaturation efficiency | Locusta migratoria manilensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Locusta migratoria manilensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-arginine | - |
Locusta migratoria manilensis | ADP + Nomega-phospho-L-arginine | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
synthesis direction of phosphoarginine and ADP | Locusta migratoria manilensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 70 | 10°C: about 55% of maximal activity, 70°C: about 40% of maximal activity, synthesis direction of phosphoarginine and ADP | Locusta migratoria manilensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 159.4 | - |
ATP | - |
Locusta migratoria manilensis | |
| 159.4 | - |
L-arginine | - |
Locusta migratoria manilensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
synthesis direction of phosphoarginine and ADP | Locusta migratoria manilensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10.5 | pH 7.0: about 40% of maximal activity, pH 10.5: about 35% of maximal activity, synthesis direction of phosphoarginine and ADP | Locusta migratoria manilensis |
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