Application | Comment | Organism |
---|---|---|
medicine | this enzyme as a possible novel chemotherapy target for VLM syndrome in humans | Toxocara canis |
Cloned (Comment) | Organism |
---|---|
cloned it in pMAL plasmid and expressed it in Escherichia coli as a fusion protein with maltose-binding protein | Toxocara canis |
expressed in Escherichia coli as a fusion protein with maltose-binding protein | Toxocara canis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-Arg | - |
Toxocara canis | |
0.12 | - |
L-Arg | in 100 mM Tris-HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, 5 mM NADH, at 25°C | Toxocara canis | |
0.3 | - |
ATP | - |
Toxocara canis | |
0.3 | - |
ATP | in 100 mM Tris-HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, 5 mM NADH, at 25°C | Toxocara canis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | presence of a signal targeting peptide presumably targeting this protein to cytosol or endoplasmic reticulum | Toxocara canis | 5829 | - |
endoplasmic reticulum | presence of a signal targeting peptide presumably targeting this protein to cytosol or endoplasmic reticulum | Toxocara canis | 5783 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45376 | - |
x * 45376, calculated from sequence | Toxocara canis |
45380 | - |
calculated from sequence of cDNA | Toxocara canis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxocara canis | A7YVI5 | - |
- |
Purification (Comment) | Organism |
---|---|
amylose resin column chromatography | Toxocara canis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg | - |
Toxocara canis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40.3 | - |
- |
Toxocara canis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Toxocara canis | ADP + Nomega-phospho-L-Arg | - |
? | |
ATP + L-Arg | synergism for substrate binding | Toxocara canis | ADP + omega-N-phospho-L-Arg | - |
r | |
additional information | very little activity for 9.5 mM D-arginine and no activity for creatine, glycocyamine, and taurocyamine substrates examined at the final substrate concentration of 2.38 mM | Toxocara canis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45376, calculated from sequence | Toxocara canis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29.18 | - |
ATP | - |
Toxocara canis | |
29.18 | - |
L-Arg | - |
Toxocara canis | |
81 | - |
L-Arg | in 100 mM Tris-HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, 5 mM NADH, at 25°C | Toxocara canis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Toxocara canis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Toxocara canis | calculated from sequence | - |
8.28 |
Toxocara canis | calculated from sequence of cDNA | - |
8.38 |