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Literature summary for 2.7.3.3 extracted from
- Cloning, expression, purification, and characterization of arginine kinase from Locusta migratoria manilensis (2007), Comp. Biochem. Physiol. B Biochem. Mol. Biol., 148, 355-362.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Locusta migratoria manilensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.951 | - |
L-Arg | - |
Locusta migratoria manilensis |  |
| 1.27 | - |
ATP | - |
Locusta migratoria manilensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Locusta migratoria manilensis | A6M9J4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephadex G-75 gel filtration | Locusta migratoria manilensis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| addition of 1% (v/v) Tween-20 and 0.6% (w/v) SDS to the dilution system leads to higher renaturation efficiency | Locusta migratoria manilensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 220 | - |
supernatant | Locusta migratoria manilensis |
| 390.5 | - |
after purification | Locusta migratoria manilensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Arg | - |
Locusta migratoria manilensis | ADP + Nomega-phospho-L-Arg | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP:L-arginine phosphotransferase | - |
Locusta migratoria manilensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Locusta migratoria manilensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.8 | - |
L-Arg | - |
Locusta migratoria manilensis | |
| 159.4 | - |
L-Arg | - |
Locusta migratoria manilensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Locusta migratoria manilensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Locusta migratoria manilensis | |
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Release 2023.1 (January 2023)
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