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Literature summary for 2.7.3.3 extracted from

  • Suzuki, T.; Tomoyuki, T.; Uda, K.
    Kinetic properties and structural characteristics of an unusual two-domain arginine kinase of the clam Corbicula japonica (2003), FEBS Lett., 533, 95-98.
    View publication on PubMed
Search for more literature for 2.7.3.3

Cloned(Commentary)

Cloned (Comment) Organism
domain 2 is separated from the two-domain enzyme and expressed in Escherichia coli, domain 2 still exhibits activity. Expression of mutants of domain 2 in Escherichia coli: H60G, H60R and D197G Corbicula japonica

Protein Variants

Protein Variants Comment Organism
D197G mutant of domain 2, affinity for Arg in mutant enzyme is reduced considerably, accompanied by a decrease in Vmax Corbicula japonica
H60G mutant of domain 2, affinity for Arg in mutant enzyme is reduced considerably, accompanied by a decrease in Vmax Corbicula japonica
H60R mutant of domain 2, affinity for Arg in mutant enzyme is reduced considerably, accompanied by a decrease in Vmax Corbicula japonica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
 L-Arg 37°C, domain 2 Corbicula japonica
0.42
-
 L-Arg 37°C, two-domain enzyme Corbicula japonica
0.46
-
 ATP 37°C, two-domain enzyme Corbicula japonica
0.52
-
 L-Arg 37°C, D197G mutant enzyme of domain 2 Corbicula japonica
0.97
-
 ATP 37°C, domain 2 Corbicula japonica
1.02
-
 L-Arg 37°C, H60R mutant of domain 2 Corbicula japonica
3.6
-
 L-Arg 37°C, H60G mutant of domain 2 Corbicula japonica

Organism

Organism UniProt Comment Textmining
Corbicula japonica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg
-
 Corbicula japonica ADP + Nomega-phospho-L-Arg
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.92
-
 ATP 25°C, H60G mutant of domain 2 Corbicula japonica
8.92
-
 L-Arg 25°C, H60G mutant of domain 2 Corbicula japonica
9.53
-
 ATP 25°C, D197G mutant of domain 2 Corbicula japonica
9.53
-
 L-Arg 25°C, D197G mutant of domain 2 Corbicula japonica
18.1
-
 ATP 25°C, H60R mutant of domain 2 Corbicula japonica
18.1
-
 L-Arg 25°C, H60R mutant of domain 2 Corbicula japonica
44.7
-
 ATP 25°C, domain 2 Corbicula japonica
44.7
-
 L-Arg 25°C, domain 2 Corbicula japonica
73.3
-
 ATP 25°C, two-domain enzyme Corbicula japonica
73.3
-
 L-Arg 25°C, two-domain enzyme Corbicula japonica