Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Sthenoteuthis oualaniensis | |
Cl- | - |
Sthenoteuthis oualaniensis | |
Cu2+ | - |
Sthenoteuthis oualaniensis | |
Fe2+ | - |
Sthenoteuthis oualaniensis | |
K+ | 200 mM, 50% inhibition | Sthenoteuthis oualaniensis | |
Na+ | 200 mM, 50% inhibition | Sthenoteuthis oualaniensis | |
NADH | noncompetitive | Sthenoteuthis oualaniensis | |
NH4+ | 200 mM, 50% inhibition | Sthenoteuthis oualaniensis | |
SO42- | - |
Sthenoteuthis oualaniensis | |
Zn2+ | - |
Sthenoteuthis oualaniensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
ADP | pH 7.2, 25°C | Sthenoteuthis oualaniensis | |
0.8 | - |
ATP | pH 7.6, 25°C | Sthenoteuthis oualaniensis | |
1 | - |
L-Arg | pH 7.6, 25°C | Sthenoteuthis oualaniensis | |
3.5 | - |
Nomega-phospho-L-Arg | pH 7.2, 25°C | Sthenoteuthis oualaniensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 4% of the activation with Mg2+ | Sthenoteuthis oualaniensis | |
Mg2+ | Km: 0.6 mM | Sthenoteuthis oualaniensis | |
Mg2+ | divalent cation requirement is satisfied by Mg2+ or Mn2+. Km-value for Mg2+: 0.6 mM | Sthenoteuthis oualaniensis | |
Mg2+ | highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate | Sthenoteuthis oualaniensis | |
Mn2+ | Km: 0.08 mM | Sthenoteuthis oualaniensis | |
Mn2+ | divalent cation requirement is satisfied by Mg2+ or Mn2+. Km-value for Mn2+: 0.08 mM | Sthenoteuthis oualaniensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Sthenoteuthis oualaniensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | Sthenoteuthis oualaniensis | - |
ADP + Nomega-phospho-L-Arg | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sthenoteuthis oualaniensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sthenoteuthis oualaniensis |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Sthenoteuthis oualaniensis | ADP + Nomega-phospho-L-Arg | - |
? | |
ATP + L-Arg | - |
Sthenoteuthis oualaniensis | ADP + Nomega-phospho-L-Arg | - |
r | |
GDP + Nomega-phospho-L-Arg | 10% of the activity with ADP | Sthenoteuthis oualaniensis | GTP + L-Arg | - |
? | |
UDP + Nomega-phospho-L-Arg | 10% of the activity with ADP | Sthenoteuthis oualaniensis | UTP + L-Arg | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Sthenoteuthis oualaniensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
synthesis of ATP, 20 mM phosphate buffer | Sthenoteuthis oualaniensis |
6.9 | - |
synthesis of ATP, 20 mM Tris-HCl buffer | Sthenoteuthis oualaniensis |
8.2 | - |
synthesis of N-phospho-L-arginine, 20 mM Tris-HCl buffer or 20 mM phosphate buffer | Sthenoteuthis oualaniensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.9 | 6.8 | pH 5.9: about 35% of maximal activity, pH 6.8: about 55% of maximal activity, synthesis of ATP, 20 mM phosphate buffer | Sthenoteuthis oualaniensis |
6.5 | 7.3 | pH 6.5: about 45% of maximal activity, pH 9.0: about 45% of maximal activity, synthesis of ATP, 20 mM Tris-HCl buffer | Sthenoteuthis oualaniensis |
7.5 | 9 | about 50% of maximal activity at pH 7.5 and pH 9.0, synthesis of N-phospho-L-arginine, 20 mM Tris-HCl buffer | Sthenoteuthis oualaniensis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
Zn2+ | - |
Sthenoteuthis oualaniensis | |
0.15 | - |
NADH | - |
Sthenoteuthis oualaniensis | |
0.5 | - |
Cu2+ | - |
Sthenoteuthis oualaniensis | |
0.85 | - |
Ca2+ | - |
Sthenoteuthis oualaniensis | |
1.5 | - |
Fe2+ | - |
Sthenoteuthis oualaniensis | |
100 | - |
SO42- | - |
Sthenoteuthis oualaniensis |