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Literature summary for 2.7.3.2 extracted from

  • Wang, W.; Lee, J.; Hao, H.; Park, Y.D.; Qian, G.Y.
    Hydrogen peroxide (H2O2) irreversibly inactivates creatine kinase from Pelodiscus sinensis by targeting the active site cysteine (2017), Int. J. Biol. Macromol., 105, 1595-1601 .
    View publication on PubMed
Search for more literature for 2.7.3.2

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) less than 5% residual activity at 0.1 mM Pelodiscus sinensis
DTNB
-
 Pelodiscus sinensis
H2O2 irreversible inhibition. H2O2 interacts with the ADP binding region of the active site of the enzyme. Enzymatic activity is rapidly abolished with less than 1 mM H2O2. Any residual activity is completely lost at an H2O2 concentration of 2-10 mM; irreversible inhibition. The enzyme activity rapidly abolishes with less than 1 mM H2O2. Any residual activity is completely lost at an H2O2 concentration of 2-10 mM. Adding reducing agents such as 2 mM dithiothreitol, 4 mM N-acetyl-l-cysteine, or 4 mM reduced L-glutathione before H2O2 treatment prevents against inactivation caused by 0.5 mM H2O2. However, if antioxidants are added 1 h after addition of 0.5 mM H2O2, no recovery is observed compared with the H2O2-treated group Pelodiscus sinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Pelodiscus sinensis 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pelodiscus sinensis
Mg2+ required, 5 mM used in assay conditions Pelodiscus sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + creatine Pelodiscus sinensis
-
 ADP + phosphocreatine
-
 ?

Organism

Organism UniProt Comment Textmining
Pelodiscus sinensis
-
-
-
Pelodiscus sinensis A0A0G3FEK6
-
-

Purification (Commentary)

Purification (Comment) Organism
cellulose DE-52 column chromatography and Sephacryl S-200 gel filtration Pelodiscus sinensis

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Pelodiscus sinensis
-
skeletal muscle
-
 Pelodiscus sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + creatine
-
 Pelodiscus sinensis ADP + phosphocreatine
-
 ?

Synonyms

Synonyms Comment Organism
CKM
-
 Pelodiscus sinensis
muscle type creatine kinase
-
 Pelodiscus sinensis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Pelodiscus sinensis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.25
-
 in 50 mM Tris at pH 8.0 and 25°C Pelodiscus sinensis H2O2
0.25
-
 at pH 9.0 and 20°C Pelodiscus sinensis H2O2