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Literature summary for 2.7.3.2 extracted from

  • de Andrade, R.B.; Gemelli, T.; Guerra, R.B.; Funchal, C.; Wannmacher, C.M.
    Kinetic studies on the inhibition of creatine kinase activity by 3-butyl-1-phenyl-2-(phenyltelluro)oct-en-1-one in the cerebral cortex of rats (2012), Food Chem. Toxicol., 50, 3468-3474.
    View publication on PubMed
Search for more literature for 2.7.3.2

Inhibitors

Inhibitors Comment Organism Structure
(2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one organotellurium inhibits creatine kinase activity by two different mechanisms: competition with ADP and oxidation of critical sulfhydryl groups for the functioning of the enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.61
-
 phosphocreatine 0.005 mM organotellurium, preincubation time 5 min, mitochondrial fraction, including reduced glutathione (GSH), pH 7.5, 37°C Rattus norvegicus
0.67
-
 phosphocreatine no organotellurium, preincubation time 5 min, mitochondrial fraction, including reduced glutathione (GSH), pH 7.5, 37°C Rattus norvegicus
0.7
-
 phosphocreatine 0.020 mM organotellurium, preincubation time 5 min, mitochondrial fraction, including reduced glutathione (GSH), pH 7.5, 37°C Rattus norvegicus
0.87
-
 phosphocreatine no organotellurium, preincubation time 5 min, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
1.11
-
 phosphocreatine no organotellurium, preincubation time 5 min, cytosolic fraction, including reduced glutathione (GSH), pH 7.5, 37°C Rattus norvegicus
1.15
-
 phosphocreatine 0.005 mM organotellurium, preincubation time 5 min, cytosolic fraction, including reduced glutathione (GSH), pH 7.5, 37°C Rattus norvegicus
1.32
-
 phosphocreatine 0.005 mM organotellurium, preincubation time 5 min, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
1.34
-
 phosphocreatine no organotellurium, preincubation time 5 min, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
1.37
-
 ATP 0.020 mM organotellurium, preincubation time 5 min, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
1.38
-
 ATP 0.005 mM organotellurium, preincubation time 5 min, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
1.41
-
 phosphocreatine 0.020 mM organotellurium, preincubation time 5 min, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
1.72
-
 phosphocreatine 0.020 mM organotellurium, preincubation time 5 min, cytosolic fraction, including reduced glutathione (GSH), pH 7.5, 37°C Rattus norvegicus
1.73
-
 phosphocreatine 0.005 mM organotellurium, preincubation time 5 min, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
1.75
-
 ATP no organotellurium, preincubation time 30 min, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
1.94
-
 ATP 0.005 mM organotellurium, preincubation time 30 min, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
2.03
-
 ATP 0.020 mM organotellurium, preincubation time 30 min, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
2.04
-
 phosphocreatine 0.020 mM organotellurium, preincubation time 5 min, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
2.1
-
 ATP 0.005 mM organotellurium, preincubation time 30 min, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
2.1
-
 ATP no organotellurium, preincubation time 5 min, mitochondrial fraction, including reduced glutathione (GSH), pH 7.5, 37°C Rattus norvegicus
2.49
-
 ATP 0.020 mM organotellurium, preincubation time 30 min, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
2.82
-
 ATP no organotellurium, preincubation time 5 min, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
2.84
-
 ATP 0.005 mM organotellurium, preincubation time 5 min, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
3.15
-
 ATP no organotellurium, preincubation time 30 min, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
3.19
-
 ATP no organotellurium, preincubation time 5 min, cytosolic fraction, including reduced glutathione (GSH), pH 7.5, 37°C Rattus norvegicus
3.26
-
 phosphocreatine 0.005 mM organotellurium, preincubation time 30 min, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
3.34
-
 phosphocreatine 0.020 mM organotellurium, preincubation time 30 min, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
3.54
-
 phosphocreatine no organotellurium, preincubation time 30 min, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
3.55
-
 ATP no organotellurium, preincubation time 5 min, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
3.92
-
 ATP 0.020 mM organotellurium, preincubation time 5 min, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
5.3 6 ATP 0.005 mM organotellurium, preincubation time 5 min, mitochondrial fraction, including reduced glutathione (GSH), pH 7.5, 37°C Rattus norvegicus
5.77
-
 phosphocreatine 0.005 mM organotellurium, preincubation time 30 min, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
6.43
-
 phosphocreatine 0.020 mM organotellurium, preincubation time 30 min, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
6.68
-
 ATP 0.005 mM organotellurium, preincubation time 5 min, cytosolic fraction, including reduced glutathione (GSH), pH 7.5, 37°C Rattus norvegicus
8.97
-
 phosphocreatine no organotellurium, preincubation time 30 min, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
17.52
-
 ATP 0.020 mM organotellurium, preincubation time 5 min, cytosolic fraction, including reduced glutathione (GSH), pH 7.5, 37°C Rattus norvegicus
20
-
 ATP 0.020 mM organotellurium, preincubation time 5 min, mitochondrial fraction, including reduced glutathione (GSH), pH 7.5, 37°C Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphocreatine
-
 Rattus norvegicus ATP + creatine
-
 ?

Synonyms

Synonyms Comment Organism
creatine kinase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.37
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.005 mM organotellurium, preincubation time 5 min, ADP variable, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
2.53
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.005 mM organotellurium, preincubation time 5 min including reduced glutathione (GSH), ADP variable, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
5.02
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.005 mM organotellurium, preincubation time 5 min including reduced glutathione (GSH), ADP variable, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
5.46
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.005 mM organotellurium, preincubation time 5 min, phosphocreatine variable, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
5.67
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.020 mM organotellurium, preincubation time 5 min, ADP variable, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
5.78
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.020 mM organotellurium, preincubation time 5 min including reduced glutathione (GSH), ADP variable, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
6.49
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.005 mM organotellurium, preincubation time 5 min, phosphocreatine variable, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
7.1
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.020 mM organotellurium, preincubation time 30 min, ADP variable, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
7.18
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.020 mM organotellurium, preincubation time 30 min, phosphocreatine variable, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
7.5
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.020 mM organotellurium, preincubation time 30 min, ADP variable, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
7.56
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.020 mM organotellurium, preincubation time 30 min, phosphocreatine variable, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
8
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.005 mM organotellurium, preincubation time 30 min, phosphocreatine variable, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
8.12
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.005 mM organotellurium, preincubation time 30 min, ADP variable, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
8.2
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.005 mM organotellurium, preincubation time 30 min, phosphocreatine variable, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
8.22
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.005 mM organotellurium, preincubation time 30 min, ADP variable, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
8.69
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.005 mM organotellurium, preincubation time 5 min including reduced glutathione (GSH), phosphocreatine variable, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
8.76
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.005 mM organotellurium, preincubation time 5 min including reduced glutathione (GSH), phosphocreatine variable, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
9.65
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.020 mM organotellurium, preincubation time 5 min including reduced glutathione (GSH), ADP variable, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
10.25
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.020 mM organotellurium, preincubation time 5 min, phosphocreatine variable, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
10.55
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.005 mM organotellurium, preincubation time 5 min, ADP variable, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
15.45
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.020 mM organotellurium, preincubation time 5 min, ADP variable, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
16
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.020 mM organotellurium, preincubation time 5 min, phosphocreatine variable, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus
16.15
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.020 mM organotellurium, preincubation time 5 min including reduced glutathione (GSH), phosphocreatine variable, mitochondrial fraction, pH 7.5, 37°C Rattus norvegicus
16.46
-
 (2Z)-3-butyl-1-phenyl-2-(phenyltellanyl)oct-2-en-1-one 0.020 mM organotellurium, preincubation time 5 min including reduced glutathione (GSH), phosphocreatine variable, cytosolic fraction, pH 7.5, 37°C Rattus norvegicus