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Literature summary for 2.7.3.2 extracted from
- Heterogeneity of Escherichia coli-expressed human muscle creatine kinase (2006), IUBMB Life, 58, 421-428.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | expression of enzyme in Escherichia coli results in four different isoforms having similar kinetic parameters and identical bands on SDS-PAGE but different anodal mobility on non-denaturing gels. Cause of isoform formation may be deamidation of asparagine or glutamine residues | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.34 | - |
ATP | isoform III isolated after expression in Escherichia coli, pH 8.0, 30°C | Homo sapiens |  |
| 0.36 | - |
ATP | isoform II isolated after expression in Escherichia coli, pH 8.0, 30°C | Homo sapiens | |
| 0.4 | - |
ATP | wild-type and isoform I isolated after expression in Escherichia coli, pH 8.0, 30°C | Homo sapiens | |
| 0.43 | - |
ATP | isoform IV isolated after expression in Escherichia coli, pH 8.0, 30°C | Homo sapiens | |
| 2 | 3 | Creatine | isoform I isolated after expression in Escherichia coli, pH 8.0, 30°C | Homo sapiens | |
| 19 | - |
Creatine | isoform II isolated after expression in Escherichia coli, pH 8.0, 30°C | Homo sapiens | |
| 20 | - |
Creatine | isoform III isolated after expression in Escherichia coli, pH 8.0, 30°C | Homo sapiens | |
| 21 | - |
Creatine | wild-type and isoform IV isolated after expression in Escherichia coli, pH 8.0, 30°C | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
expression in Escherichia coli | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | - |
Homo sapiens | ADP + creatine phosphate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 76 | - |
ATP | isoform II isolated after expression in Escherichia coli, pH 8.0, 30°C | Homo sapiens | |
| 88 | - |
ATP | isoform IV isolated after expression in Escherichia coli, pH 8.0, 30°C | Homo sapiens | |
| 115 | - |
ATP | isoform III isolated after expression in Escherichia coli, pH 8.0, 30°C | Homo sapiens | |
| 143 | - |
ATP | wild-type, pH 8.0, 30°C | Homo sapiens | |
| 155 | - |
ATP | isoform I isolated after expression in Escherichia coli, pH 8.0, 30°C | Homo sapiens | |
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Release 2023.1 (January 2023)
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