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Literature summary for 2.7.2.8 extracted from

  • Beez, S.; Fokina, O.; Herrmann, C.; Forchhammer, K.
    N-acetyl-L-glutamate kinase (NAGK) from oxygenic phototrophs: P(II) signal transduction across domains of life reveals novel insights in NAGK control (2009), J. Mol. Biol., 389, 748-758.
    View publication on PubMed
Search for more literature for 2.7.2.8

Activating Compound

Activating Compound Comment Organism Structure
PII protein
-
 Synechococcus elongatus
PII protein
-
 Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Synechococcus elongatus
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate complete inhibition at 0.5 mM Arabidopsis thaliana
L-arginine complete inhibition at 3-5 mM, PII-mediated relief from L-arginine inhibition is antagonized by 2-oxoglutarate Arabidopsis thaliana
L-arginine complete inhibition at 0.1-1.0 mM, PII-mediated relief from L-arginine inhibition is antagonized by 2-oxoglutarate Synechococcus elongatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 ATP in the absence of protein PII, in 50 mM imidazole, pH 7.5 Arabidopsis thaliana
0.4
-
 ATP in the absence of protein PII, in 50 mM imidazole, pH 7.5 Arabidopsis thaliana
0.6
-
 ATP in the absence of protein PII, in 50 mM imidazole, pH 7.5 Synechococcus elongatus
0.85
-
 N-acetyl-L-glutamate in the absence of protein PII, in 50 mM imidazole, pH 7.5 Arabidopsis thaliana
0.87
-
 N-acetyl-L-glutamate in the absence of protein PII, in 50 mM imidazole, pH 7.5 Arabidopsis thaliana
1.1
-
 ATP in the absence of protein PII, in 50 mM imidazole, pH 7.5 Synechococcus elongatus
3.1
-
 N-acetyl-L-glutamate in the absence of protein PII, in 50 mM imidazole, pH 7.5 Synechococcus elongatus
7.4
-
 N-acetyl-L-glutamate in the absence of protein PII, in 50 mM imidazole, pH 7.5 Synechococcus elongatus
85
-
 N-acetyl-L-glutamate in the presence of 1 mM L-arginine, in 50 mM imidazole, pH 7.5 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-L-glutamate Synechococcus elongatus
-
 ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
ATP + N-acetyl-L-glutamate Arabidopsis thaliana
-
 ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SCL7
-
-
Synechococcus elongatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Synechococcus elongatus
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-L-glutamate
-
 Synechococcus elongatus ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
ATP + N-acetyl-L-glutamate
-
 Arabidopsis thaliana ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
N-acetyl-L-glutamate kinase
-
 Synechococcus elongatus
N-acetyl-L-glutamate kinase
-
 Arabidopsis thaliana
NagK
-
 Synechococcus elongatus
NagK
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.1
-
 N-acetyl-L-glutamate in the absence of protein PII, in 50 mM imidazole, pH 7.5 Synechococcus elongatus
40.4
-
 N-acetyl-L-glutamate in the presence of protein PII, in 50 mM imidazole, pH 7.5 Synechococcus elongatus
115
-
 N-acetyl-L-glutamate in the presence of 1 mM L-arginine, in 50 mM imidazole, pH 7.5 Arabidopsis thaliana
126.1
-
 N-acetyl-L-glutamate in the absence of protein PII, in 50 mM imidazole, pH 7.5 Arabidopsis thaliana
187.5
-
 N-acetyl-L-glutamate in the presence of protein PII, in 50 mM imidazole, pH 7.5 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Synechococcus elongatus
ATP
-
 Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.02
-
 in the absence of protein PII, in 50 mM imidazole, pH 7.5 Synechococcus elongatus L-arginine
0.19
-
 in the presence of protein PII, in 50 mM imidazole, pH 7.5 Synechococcus elongatus L-arginine
1
-
 in the absence of protein PII, in 50 mM imidazole, pH 7.5 Arabidopsis thaliana L-arginine
5.9
-
 in the presence of protein PII, in 50 mM imidazole, pH 7.5 Arabidopsis thaliana L-arginine

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3
-
 N-acetyl-L-glutamate in the presence of 1 mM L-arginine, in 50 mM imidazole, pH 7.5 Arabidopsis thaliana
1.7
-
 N-acetyl-L-glutamate in the absence of protein PII, in 50 mM imidazole, pH 7.5 Synechococcus elongatus
13
-
 N-acetyl-L-glutamate in the presence of protein PII, in 50 mM imidazole, pH 7.5 Synechococcus elongatus
145
-
 N-acetyl-L-glutamate in the absence of protein PII, in 50 mM imidazole, pH 7.5 Arabidopsis thaliana
220
-
 N-acetyl-L-glutamate in the presence of protein PII, in 50 mM imidazole, pH 7.5 Arabidopsis thaliana