Literature summary for 2.7.2.8 extracted from

Chen, Y.M.; Ferrar, T.S.; Lohmeir-Vogel, E.; Morrice, N.; Mizuno, Y.; Berenger, B.; Ng, K.K.; Muench, D.G.; Moorhead, G.B.
The PII signal transduction protein of Arabidopsis thaliana forms an arginine-regulated complex with plastid N-acetyl glutamate kinase (2006), J. Biol. Chem., 281, 5726-5733.

Search for more literature for 2.7.2.8

Activating Compound

Activating Compound	Comment	Organism	Structure
2-Ketoglutarate	only if bound to the PII protein ATP complex	Arabidopsis thaliana
PII protein	prevents inhibition by arginine	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
L-arginine	less inhibitory in the presence of the PII protein	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.74	-	ATP	without PII protein	Arabidopsis thaliana
2.03	-	ATP	in complex with PII protein	Arabidopsis thaliana
2.47	-	ATP	in complex with PII protein and 2-ketoglutarate	Arabidopsis thaliana
7.08	-	N-acetyl-L-glutamate	without PII protein	Arabidopsis thaliana
7.55	-	N-acetyl-L-glutamate	in complex with PII protein	Arabidopsis thaliana
9.45	-	N-acetyl-L-glutamate	in complex with PII protein and 2-ketoglutarate	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Arabidopsis thaliana	9507	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	mass spectrometry	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9SCL7	-	-

Purification (Commentary)

Purification (Comment)	Organism
affinity chromatography and gel filtration by binding to the PII protein, Ni-nitrilotriacetic acid agarose to purify the recombinant protein	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + N-acetyl-L-glutamate	-	Arabidopsis thaliana	ADP + N-acetyl-L-glutamate 5-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
acetylglutamate kinase	-	Arabidopsis thaliana
N-acetylglutamate kinase	-	Arabidopsis thaliana
NagK	-	Arabidopsis thaliana
NAGS-K	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)