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Literature summary for 2.7.2.8 extracted from

  • Fernandez-Murga, M.L.; Gil-Ortiz, F.; Llacer, J.L.; Rubio, V.
    Arginine biosynthesis in Thermotoga maritima: characterization of the arginine-sensitive N-acetyl-L-glutamate kinase (2004), J. Bacteriol., 186, 6142-6149.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
L-arginine pH and temperature dependent, sigmoidal dependence on concentration of arginine, Hill coefficient of 4, 1 mM, 37°C, 95% inhibition Thermotoga maritima
L-arginine methyl ester 1 mM, 80% inhibition Thermotoga maritima
additional information not inhibitory: D-arginine, agmatine, citrulline, L-canavanine, L-lysine, L-ornithine, guanidinium ions, urea at 1 mM, 37°C Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30341
-
6 * 30341, MALDI-TOF MS, 6 * 30344, calculated Thermotoga maritima
30344
-
6 * 30341, MALDI-TOF MS, 6 * 30344, calculated Thermotoga maritima
180000
-
gel filtration Pseudomonas aeruginosa
180000
-
gel filtration Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
recombinant enzyme
-
Thermotoga maritima
-
recombinant enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
45
-
37°C, pH 7.5 Thermotoga maritima
130
-
37°C, pH 7.5 Pseudomonas aeruginosa
677
-
80°C, pH 7.5 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-L-glutamate
-
Pseudomonas aeruginosa ADP + N-acetyl-L-glutamate 5-phosphate
-
?
ATP + N-acetyl-L-glutamate
-
Thermotoga maritima ADP + N-acetyl-L-glutamate 5-phosphate
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 30341, MALDI-TOF MS, 6 * 30344, calculated Thermotoga maritima

Synonyms

Synonyms Comment Organism
NagK
-
Pseudomonas aeruginosa
NagK
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
30 min, complete inactivation Pseudomonas aeruginosa
70
-
1 h, 100% residual activity Thermotoga maritima
80
-
1 h, 80% residual activity Thermotoga maritima
85
-
1 h, 60% residual activity Thermotoga maritima