Literature summary for 2.7.2.4 extracted from

Tsujimoto, M.; Yoshida, A.; Shimizu, T.; Tomita, T.; Ohnishi, Y.; Kuzuyama, T.; Nishiyama, M.
Aspartate kinase involved in 4-hydroxy-3-nitrosobenzamide biosynthesis in Streptomyces murayamaensis (2016), Biosci. Biotechnol. Biochem., 80, 2255-2263 .

Search for more literature for 2.7.2.4

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL and BL21 (DE3) cells as well as in Streptomyces lividans strain TK23	Streptomyces murayamaensis

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the enzyme is insensitive to lysine, threonine, methionine, and isoleucine. Furthermore, the addition of lysine + threonine or lysine + threonine + methionine + isoleucine does not affect its activity. The enzyme is not inhibited by 4-hydroxy-3-nitrosobenzamide (0.1 mM), and 3-amino-4-hydroxybenzoic acid (1 mM)	Streptomyces murayamaensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.9	-	L-aspartate	at pH 8.0 and 30Â°C	Streptomyces murayamaensis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	10 mM used in assay conditions	Streptomyces murayamaensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
160000	-	gel filtration	Streptomyces murayamaensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-aspartate	Streptomyces murayamaensis	-	ADP + 4-phospho-L-aspartate	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces murayamaensis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, and Superdex 200 gel filtration	Streptomyces murayamaensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-aspartate	-	Streptomyces murayamaensis	ADP + 4-phospho-L-aspartate	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	2 * 44000 + 2 * 22000, SDS-PAGE	Streptomyces murayamaensis

Synonyms

Synonyms	Comment	Organism
nspJ	-	Streptomyces murayamaensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
17.4	-	L-aspartate	at pH 8.0 and 30Â°C	Streptomyces murayamaensis

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in the biosynthesis of 4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis

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Release 2023.1 (January 2023)