Literature summary for 2.7.2.4 extracted from

Li, C.C.; Yang, M.J.; Liu, L.; Li, T.; Peng, C.T.; He, L.H.; Song, Y.J.; Zhu, Y.B.; Shen, Y.L.; Yang, J.; Zhao, N.L.; Zhao, C.; Zhou, Q.X.; Li, H.; Kang, M.; Tong, A.P.; Tang, H.; Bao, R.
Mechanistic insights into the allosteric regulation of Pseudomonas aeruginosa aspartate kinase (2018), Biochem. J., 475, 1107-1119 .

Search for more literature for 2.7.2.4

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 0.4 M ammonium sulfate, 0.1 M sodium citrate tribasic dihydrate (pH 5.6) and 0.9 M lithium sulfate monohydrate	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
D182/R184A	the mutations decrease the enzyme activities to about 65%	Pseudomonas aeruginosa
G152L	the mutant shows about 35% activity compared to the wild type enzyme	Pseudomonas aeruginosa
H399A	the mutant shows wild type activity	Pseudomonas aeruginosa
N371A/I372A	the mutations do not affect the ATP binding with threonine	Pseudomonas aeruginosa
R150A	the mutant shows about 30% activity compared to the wild type enzyme	Pseudomonas aeruginosa
S12C/S231C	the mutant shows wild type activity	Pseudomonas aeruginosa
S378A/E202A	the mutant shows slightly increased activity (about 108%) compared to the wild type enzyme	Pseudomonas aeruginosa
S378A/E202A	the mutations do not affect the ATP binding with threonine	Pseudomonas aeruginosa
V357/M351A	the mutant shows reduced activity compared to the wild type enzyme	Pseudomonas aeruginosa

Inhibitors

Inhibitors	Comment	Organism	Structure
lysine	-	Pseudomonas aeruginosa
threonine	-	Pseudomonas aeruginosa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	10 mM used in assay conditions	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-aspartate	Pseudomonas aeruginosa	-	ADP + 4-phospho-L-aspartate	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	O69077	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography and Superdex 200 gel filtration	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-aspartate	-	Pseudomonas aeruginosa	ADP + 4-phospho-L-aspartate	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	x-ray crystallography	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
aspartokinase	-	Pseudomonas aeruginosa
lysC	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)