Cloned (Comment) | Organism |
---|---|
into the NdeI/HindIII site of pET26b(+) and introduced into Escherichia coli BL21-CodonPlus(DE3)-RIL cells | Thermus thermophilus |
into vector pET26b(+) and introduced into Escherichia coli BL21-CodonPlus(DE3)-RIL cells | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
by hanging-drop vapour-diffusion method, regulatory subunit in the presence of the inhibitor threonine, to 2.15 A resolution, crystal belongs to the cubic space group P4332 or P4132, with unit-cell parameters a = b = c = 141.8 A | Thermus thermophilus |
by the hanging-drop vapour-diffusion method using Crystal Screen kits, the regulatory subunit (the beta subunit of Thermus thermophilus AK) is crystallized in the presence of the inhibitor threonine. Diffraction data are collected to 2.15 A at a synchrotron source. The crystal belongs to the cubic space group P4332 or P4132, with unit-cell parameters a = b = c = 141.8 A. | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-threonine | feedback inhibition by the end product | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17720 | - |
full-length beta subunit (Met1ΒAla161) with a His6-tag extension at the C-terminal end, calculated | Thermus thermophilus |
17720 | - |
beta subunit (Met1-Ala161), calculated from nucleotide sequence | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | P61489 | - |
- |
Thermus thermophilus | P61489 | expression in Escherichia coli BL21-CodonPlus(DE3)-RIL cells | - |
Purification (Comment) | Organism |
---|---|
by nickel-affinity chromatography and gel filtration, to more than 95% homogeneity | Thermus thermophilus |
nickel-affinity chromatography, gel-filtration FPLC column, more than 95% homogeneity of the purified subunit is verified by SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
AK | - |
Thermus thermophilus |
aspartate kinase | - |
Thermus thermophilus |