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Literature summary for 2.7.2.4 extracted from

  • Faehnle, C.R.; Liu, X.; Pavlovsky, A.; Viola, R.E.
    The initial step in the archaeal aspartate biosynthetic pathway catalyzed by a monofunctional aspartokinase (2006), Acta Crystallogr. Sect. F, 62, 962-966.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.4

Cloned(Commentary)

Cloned (Comment) Organism
into pET-41a expression vector and expression in Escherichia coli Rosetta (DE3) cells Methanocaldococcus jannaschii

Crystallization (Commentary)

Crystallization (Comment) Organism
by hanging-drop vapor-diffusion method, in the presence of L-aspartate and MgADP, to 2.82–2.69 A resolution, N-terminal catalytic domain (residues 2-300) and a C-terminal regulatory domain (residues 310-470) joined through a hinge region (residues 301-309) Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
L-threonine
-
 Methanocaldococcus jannaschii
additional information L-lysine has no effect on activity at concentrations up to 10 mM, not inhibited by L-isoleucine and L-methionine Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stabilizes the negative charge density on the terminal phosphoryl group and lowers the barrier towards transfer to the beta-carboxyl group of L-aspartate Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 dynamic light-scattering, addition up to 4000 mM guanidine-HCl leads to dissociation of the AK dimers into monomers Methanocaldococcus jannaschii
83000
-
 dynamic light-scattering, addition of increasing levels of guanidine-HCl up to 2000 mM causes a decrease in the AK particle size, dissociation into dimers Methanocaldococcus jannaschii
154000
-
 dynamic light-scattering, crystallization Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57991
-
-

Purification (Commentary)

Purification (Comment) Organism
by anion-exchange chromatography and gel filtration, more than 99% pure Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate
-
 Methanocaldococcus jannaschii ADP + 4-phospho-L-aspartate
-
 ?

Subunits

Subunits Comment Organism
dimer dynamic light-scattering, addition of increasing levels of guanidine-HCl up to 2000 mM Methanocaldococcus jannaschii
monomer dynamic light-scattering, addition up to 4000 mM guanidine-HCl Methanocaldococcus jannaschii
tetramer crystallography, dynamic light-scattering, organized into a dimer of dimers, dimer interface mediated through both ACT subdomains, formation of the tetramer stabilized by the interaction of complementary residues from alpha4 of the catalytic domain Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
AK
-
 Methanocaldococcus jannaschii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 70
-
 Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12
-
 L-aspartate at 37°C Methanocaldococcus jannaschii
245
-
 L-aspartate at 70°C Methanocaldococcus jannaschii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.3
-
-
 Methanocaldococcus jannaschii L-threonine