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Literature summary for 2.7.2.4 extracted from
- Overproduction, purification, and characterization of recombinant bifunctional threonine-sensitive aspartate kinase-homoserine dehydrogenase from Arabidopsis thaliana (2002), Protein Expr. Purif., 24, 105-110.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | enzyme does not exist in animals, good target for conception of new pesticides controlling weeds, fungi and bacteria | Arabidopsis thaliana |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Arabidopsis thaliana |
| cDNA located on chromosome 4, cloned in Escherichia coli DH5alpha, overproduced in Escherichia coli BL21pLysS | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-threonine | - |
Arabidopsis thaliana |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.5 | - |
ATP | pH 8.0, 30°C | Arabidopsis thaliana | |
| 11.6 | - |
L-aspartate | pH 8.0, 30°C | Arabidopsis thaliana | |
| 12.9 | - |
ATP | pH 8.0, 30°C, 0.5 M effector threonine | Arabidopsis thaliana | |
| 26.4 | - |
L-aspartate | pH 8.0, 30°C, 0.5 M effector threonine | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plastid | - |
Arabidopsis thaliana | 9536 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 93000 | - |
? * 93000, SDS-PAGE | Arabidopsis thaliana |
| 320000 | - |
gel filtration, in presence of 5.0 mM L-threonine | Arabidopsis thaliana |
| 470000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate | Arabidopsis thaliana | first and third step of the synthesis of methionine, catalyzed by a bifunctional enzyme | ADP + 4-phospho-L-aspartate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Arabidopsis thaliana | O81852 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native and recombinant enzyme | Arabidopsis thaliana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
forward reaction | Arabidopsis thaliana |
| 18.87 | - |
reverse reaction | Arabidopsis thaliana |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, stored frozen without noticeable loss of enzyme activity | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate | - |
Arabidopsis thaliana | ADP + 4-phospho-L-aspartate | - |
r | |
| ATP + L-aspartate | first and third step of the synthesis of methionine, catalyzed by a bifunctional enzyme | Arabidopsis thaliana | ADP + 4-phospho-L-aspartate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | ? * 93000, SDS-PAGE | Arabidopsis thaliana |
| tetramer | - |
Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AK-HSDH | - |
Arabidopsis thaliana |
| aspartokinase-homoserine dehydrogenase | - |
Arabidopsis thaliana |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Arabidopsis thaliana | theoretical pI | - |
6.13 |
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