Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-threonine | 0.01 mM, mutant G135A, activity 110%, mutant R150A, activity 115% | Thermus thermophilus |
Cloned (Comment) | Organism |
---|---|
2 subunits, alpha and beta, encoded by an inframe overlapping gene, askAB genes cloned and expressed in Escherichia coli GT3 | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
A157L | site-directed mutagenesis | Thermus thermophilus |
A189L | site-directed mutagenesis | Thermus thermophilus |
A23L | site-directed mutagenesis | Thermus thermophilus |
A42S | site-directed mutagenesis | Thermus thermophilus |
D154A | site-directed mutagenesis | Thermus thermophilus |
D154N | site-directed mutagenesis | Thermus thermophilus |
D174A | site-directed mutagenesis | Thermus thermophilus |
D182A | site-directed mutagenesis | Thermus thermophilus |
E202A | site-directed mutagenesis | Thermus thermophilus |
F136A | site-directed mutagenesis | Thermus thermophilus |
G10A | site-directed mutagenesis | Thermus thermophilus |
G135A | site-directed mutagenesis | Thermus thermophilus |
G149A | site-directed mutagenesis | Thermus thermophilus |
G152A | site-directed mutagenesis | Thermus thermophilus |
G177A | site-directed mutagenesis | Thermus thermophilus |
G73A | site-directed mutagenesis | Thermus thermophilus |
I171A | site-directed mutagenesis | Thermus thermophilus |
L148A | site-directed mutagenesis | Thermus thermophilus |
P183A | site-directed mutagenesis | Thermus thermophilus |
R150A | site-directed mutagenesis | Thermus thermophilus |
S12A | site-directed mutagenesis | Thermus thermophilus |
S153A | site-directed mutagenesis | Thermus thermophilus |
S41A | site-directed mutagenesis | Thermus thermophilus |
T238A | site-directed mutagenesis | Thermus thermophilus |
T47A | site-directed mutagenesis | Thermus thermophilus |
V39A | site-directed mutagenesis | Thermus thermophilus |
Y8A | site-directed mutagenesis | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
L-aspartate | pH 7.5, 60°C, mutant A157L | Thermus thermophilus | |
0.27 | - |
L-aspartate | pH 7.5, 60°C, mutant E202A | Thermus thermophilus | |
0.27 | - |
ATP | pH 7.5, 60°C, mutant G177A | Thermus thermophilus | |
0.29 | - |
ATP | pH 7.5, 60°C, mutant G149A | Thermus thermophilus | |
0.32 | - |
L-aspartate | pH 7.5, 60°C, mutant Y8A, S12A | Thermus thermophilus | |
0.33 | - |
L-aspartate | pH 7.5, 60°C, mutant V39A | Thermus thermophilus | |
0.34 | - |
L-aspartate | pH 7.5, 60°C, mutant G177A | Thermus thermophilus | |
0.36 | - |
L-aspartate | pH 7.5, 60°C, mutant G149A, G177A | Thermus thermophilus | |
0.37 | - |
L-aspartate | pH 7.5, 60°C, mutant I171A | Thermus thermophilus | |
0.39 | - |
L-aspartate | pH 7.5, 60°C, mutant T238A | Thermus thermophilus | |
0.41 | - |
ATP | pH 7.5, 60°C, mutant G149A | Thermus thermophilus | |
0.41 | - |
L-aspartate | pH 7.5, 60°C, mutant S41A | Thermus thermophilus | |
0.42 | - |
L-aspartate | pH 7.5, 60°C, mutant G152A, P183A | Thermus thermophilus | |
0.43 | - |
ATP | pH 7.5, 60°C, mutant D174A | Thermus thermophilus | |
0.44 | - |
ATP | pH 7.5, 60°C, mutant S153A | Thermus thermophilus | |
0.45 | - |
ATP | pH 7.5, 60°C, mutant A157L | Thermus thermophilus | |
0.45 | - |
L-aspartate | pH 7.5, 60°C, mutant A189L | Thermus thermophilus | |
0.46 | - |
ATP | pH 7.5, 60°C, mutant G150A | Thermus thermophilus | |
0.47 | - |
L-aspartate | pH 7.5, 60°C, mutant S153A | Thermus thermophilus | |
0.5 | - |
ATP | pH 7.5, 60°C, wild-type and mutant G152A | Thermus thermophilus | |
0.52 | - |
ATP | pH 7.5, 60°C, mutant S12A, G135A | Thermus thermophilus | |
0.53 | - |
L-aspartate | pH 7.5, 60°C, mutant D174A | Thermus thermophilus | |
0.56 | - |
ATP | pH 7.5, 60°C, mutant T238A | Thermus thermophilus | |
0.57 | - |
ATP | pH 7.5, 60°C, mutant F136A | Thermus thermophilus | |
0.6 | - |
ATP | pH 7.5, 60°C, mutant I171A | Thermus thermophilus | |
0.6 | - |
L-aspartate | pH 7.5, 60°C, mutant A42S | Thermus thermophilus | |
0.61 | - |
L-aspartate | pH 7.5, 60°C, mutant G73A | Thermus thermophilus | |
0.63 | - |
ATP | pH 7.5, 60°C, mutant D154N | Thermus thermophilus | |
0.65 | - |
L-aspartate | pH 7.5, 60°C, mutant A23L | Thermus thermophilus | |
0.72 | - |
ATP | pH 7.5, 60°C, mutant A23L | Thermus thermophilus | |
0.86 | - |
ATP | pH 7.5, 60°C, mutant Y8A | Thermus thermophilus | |
0.89 | - |
ATP | pH 7.5, 60°C, mutant V39A | Thermus thermophilus | |
0.97 | - |
ATP | pH 7.5, 60°C, mutant L148A | Thermus thermophilus | |
0.98 | - |
ATP | pH 7.5, 60°C, mutant A189L | Thermus thermophilus | |
1.02 | - |
ATP | pH 7.5, 60°C, mutant A42S | Thermus thermophilus | |
1.05 | - |
ATP | pH 7.5, 60°C, mutant D154A | Thermus thermophilus | |
1.2 | - |
L-aspartate | pH 7.5, 60°C, mutant P183A | Thermus thermophilus | |
1.23 | - |
L-aspartate | pH 7.5, 60°C, mutant R150A, D154A | Thermus thermophilus | |
1.28 | - |
ATP | pH 7.5, 60°C, mutant G73A | Thermus thermophilus | |
1.31 | - |
L-aspartate | pH 7.5, 60°C, D182A | Thermus thermophilus | |
1.34 | - |
L-aspartate | pH 7.5, 60°C, mutant G10A, G135A | Thermus thermophilus | |
1.39 | - |
ATP | pH 7.5, 60°C, mutant S41A | Thermus thermophilus | |
1.9 | - |
ATP | pH 7.5, 60°C, mutant P183A | Thermus thermophilus | |
2.1 | - |
L-aspartate | pH 7.5, 60°C, mutant L148A | Thermus thermophilus | |
2.1 | - |
ATP | pH 7.5, 60°C, mutant T47A | Thermus thermophilus | |
2.45 | - |
ATP | pH 7.5, 60°C, mutant D182A | Thermus thermophilus | |
3.57 | - |
ATP | pH 7.5, 60°C, mutant G10A | Thermus thermophilus | |
5.29 | - |
L-aspartate | pH 7.5, 60°C, mutant F136A | Thermus thermophilus | |
19.5 | - |
L-aspartate | pH 7.5, 60°C, mutant T47A | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
1 * 17000 + 1 * 43000, SDS-PAGE | Thermus thermophilus |
43000 | - |
1 * 17000 + 1 * 43000, SDS-PAGE | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | Thermus thermophilus | first step of the branched biosynthetic pathway for lysine, threonine, isoleucine and methionine | ADP + 4-phospho-L-aspartate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, expressed in E. coli | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
- |
Thermus thermophilus |
Storage Stability | Organism |
---|---|
4°C, purified enzyme stable for at least 2 weeks in gel filtration buffer | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Thermus thermophilus | ADP + 4-phospho-L-aspartate | - |
r | |
ATP + L-aspartate | first step of the branched biosynthetic pathway for lysine, threonine, isoleucine and methionine | Thermus thermophilus | ADP + 4-phospho-L-aspartate | - |
r |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 17000 + 1 * 43000, SDS-PAGE | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
L-aspartate | pH 7.5, 60°C, mutant S41A | Thermus thermophilus | |
0.967 | - |
L-aspartate | pH 7.5, 60°C, mutant G135A | Thermus thermophilus | |
1.83 | - |
L-aspartate | pH 7.5, 60°C, mutant R150A, D154A | Thermus thermophilus | |
2.17 | - |
L-aspartate | pH 7.5, 60°C, mutant D154N, D182A | Thermus thermophilus | |
2.83 | - |
L-aspartate | pH 7.5, 60°C, mutant G10A | Thermus thermophilus | |
3 | - |
L-aspartate | pH 7.5, 60°C, mutant D174A | Thermus thermophilus | |
4 | - |
L-aspartate | pH 7.5, 60°C, mutant E202A | Thermus thermophilus | |
4.33 | - |
L-aspartate | pH 7.5, 60°C, mutant G152A, P183A | Thermus thermophilus | |
4.67 | - |
L-aspartate | pH 7.5, 60°C, mutant A157L | Thermus thermophilus | |
6 | - |
L-aspartate | pH 7.5, 60°C, mutant A189L | Thermus thermophilus | |
6.67 | - |
L-aspartate | pH 7.5, 60°C, mutant T47A | Thermus thermophilus | |
7.33 | - |
L-aspartate | pH 7.5, 60°C, mutant T238A | Thermus thermophilus | |
10.8 | - |
L-aspartate | pH 7.5, 60°C, mutant I171A | Thermus thermophilus | |
12.3 | - |
L-aspartate | pH 7.5, 60°C, mutant S153A | Thermus thermophilus | |
13.3 | - |
L-aspartate | pH 7.5, 60°C, mutant G149A, G177A | Thermus thermophilus | |
13.8 | - |
L-aspartate | pH 7.5, 60°C, mutant Y8A | Thermus thermophilus | |
16.7 | - |
L-aspartate | pH 7.5, 60°C, mutant F136A | Thermus thermophilus | |
23.2 | - |
L-aspartate | pH 7.5, 60°C, mutant A42S | Thermus thermophilus | |
23.3 | - |
L-aspartate | pH 7.5, 60°C, mutant L148A | Thermus thermophilus | |
24.3 | - |
L-aspartate | pH 7.5, 60°C, mutant A23L | Thermus thermophilus | |
24.7 | - |
L-aspartate | pH 7.5, 60°C, mutant S12A | Thermus thermophilus | |
25 | - |
L-aspartate | pH 7.5, 60°C, wild-type | Thermus thermophilus | |
31.7 | - |
L-aspartate | pH 7.5, 60°C, mutant G73A | Thermus thermophilus | |
32 | - |
L-aspartate | pH 7.5, 60°C, mutant V39A | Thermus thermophilus |