Literature summary for 2.7.2.4 extracted from

Arevalo-Rodriguez, M.; Calderon, I.L.; Holmberg, S.
Mutations that cause threonine sensitivity identify catalytic and regulatory regions of the aspartate kinase of Saccharomyces cerevisiae (1999), Yeast, 15, 1331-1345.

Search for more literature for 2.7.2.4

Cloned(Commentary)

Cloned (Comment)	Organism
single aspartate kinase encoded by the HOM3 gene, Escherichia coli DH5alpha as host	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
A406T	site-directed mutagenesis, 30fold more strongly inhibited by threonine	Saccharomyces cerevisiae
G25D	site-directed mutagenesis, reduced affinity for its substrates aspartate and ATP	Saccharomyces cerevisiae
K26I	site-directed mutagenesis, reduced affinity for its substrates aspartate and ATP	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
L-threonine	-	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
5.4	-	ATP	mutant hom3-S45	Saccharomyces cerevisiae
10.4	-	ATP	mutant hom3-S49	Saccharomyces cerevisiae
11.3	-	L-aspartate	mutant hom3-S45	Saccharomyces cerevisiae
12.4	-	L-aspartate	mutant hom3-S49	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-aspartate	Saccharomyces cerevisiae	first step in the branched pathway leading to synthesis of threonine and methionine from aspartate	ADP + 4-phospho-L-aspartate	-	r

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	yeast	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-aspartate	-	Saccharomyces cerevisiae	ADP + 4-phospho-L-aspartate	-	r
ATP + L-aspartate	first step in the branched pathway leading to synthesis of threonine and methionine from aspartate	Saccharomyces cerevisiae	ADP + 4-phospho-L-aspartate	-	r

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Release 2023.1 (January 2023)