Literature summary for 2.7.2.4 extracted from

Zhang, W.W.; Jiang, W.H.; Zhao, G.P.; Yang, Y.L.; Chiao, J.S.
Expression in Escherichia coli, purification and kinetic analysis of the aspartokinase and aspartate semialdehyde dehydrogenase from the rifamycin SV-producing Amycolatopsis mediterranei U32 (2000), Appl. Microbiol. Biotechnol., 54, 52-58.

Search for more literature for 2.7.2.4

Cloned(Commentary)

Cloned (Comment)	Organism
operon encoding aspartokinase cloned and sequenced, introduced into auxotrophic Escherichia coli CGSC5074 and Escherichia coli X6118 and functionally expressed	Amycolatopsis mediterranei

Inhibitors

Inhibitors	Comment	Organism	Structure
L-isoleucine	repression of aspartokinase gene transcription	Amycolatopsis mediterranei
L-lysine	repression of aspartokinase gene transcription	Amycolatopsis mediterranei
L-threonine	repression of aspartokinase gene transcription	Amycolatopsis mediterranei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.3	-	ATP	-	Amycolatopsis mediterranei
3.4	-	L-aspartate	-	Amycolatopsis mediterranei

Organism

Organism	UniProt	Comment	Textmining
Amycolatopsis mediterranei	-	U32	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme, expressed in E. coli	Amycolatopsis mediterranei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-aspartate	-	Amycolatopsis mediterranei	ADP + 4-phospho-L-aspartate	-	?

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Release 2023.1 (January 2023)