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Literature summary for 2.7.2.3 extracted from
- Molecular cloning, purification and characterization of Brugia malayi phosphoglycerate kinase (2017), Protein Expr. Purif., 132, 152-163 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Brugia malayi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | 59% inhibition at 0.002 mM | Brugia malayi |  |
| albendazole | 67% inhibition at 0.03 mM | Brugia malayi | |
| clorsulon | competitive inhibitor, 85% inhibition at 0.015 mM | Brugia malayi | |
| iodoacetamide | 83% inhibition at 2 mM | Brugia malayi | |
| ivermectin | 41% inhibition at 0.04 mM | Brugia malayi | |
| N-ethylmaleimide | 59% inhibition at 0.015 mM | Brugia malayi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.961 | - |
ATP | at pH 8.5 and 25°C | Brugia malayi | |
| 1.77 | - |
3-phospho-D-glycerate | at pH 8.5 and 25°C | Brugia malayi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10 mM used in assay conditions | Brugia malayi | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | Brugia malayi | - |
ADP + 3-phospho-D-glyceroyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brugia malayi | A0A0H5S3P5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Brugia malayi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Brugia malayi | ADP + 3-phospho-D-glyceroyl phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Brugia malayi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-phosphoglycerate kinase | - |
Brugia malayi |
| PGK | - |
Brugia malayi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Brugia malayi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Brugia malayi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | the enzyme is active in the pH range 8.0-8.5 with maximal activity at pH 8.5 and a rapid decline in the activity outside this pH range | Brugia malayi |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00188 | - |
clorsulon | at pH 8.5 and 25°C | Brugia malayi | |
| 0.03539 | - |
albendazole | at pH 8.5 and 25°C | Brugia malayi | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0008 | - |
at pH 8.5 and 25°C | Brugia malayi | clorsulon | |
| 0.0018 | - |
at pH 8.5 and 25°C | Brugia malayi | 5,5'-dithiobis(2-nitrobenzoic acid) | |
| 0.01 | - |
at pH 8.5 and 25°C | Brugia malayi | N-ethylmaleimide | |
| 0.02 | - |
at pH 8.5 and 25°C | Brugia malayi | albendazole | |
| 0.05 | - |
at pH 8.5 and 25°C | Brugia malayi | ivermectin | |
| 1.4 | - |
at pH 8.5 and 25°C | Brugia malayi | iodoacetamide | |
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