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Literature summary for 2.7.2.3 extracted from

  • Kouril, T.; Esser, D.; Kort J.; Westerhoff, H.V.; Siebers, B.; Snoep, J.L.
    Intermediate instability at high temperature leads to low pathway efficiency for an in vitro reconstituted system of gluconeogenesis in Sulfolobus solfataricus (2013), FEBS J., 280, 4666-4680.
    View publication on PubMed
Search for more literature for 2.7.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the enzyme shows hyperbolic saturation kinetics for 3-phospho-D-glycerate, ATP and 3-phospho-D-glyceroyl phosphate. For ADP it shows a high affinity binding for the reaction in the reverse direction and a low affinity inhibitory effect for the forward as well as the reverse reaction Saccharolobus solfataricus
0.085
-
 ADP 70°C, pH 6.5 Saccharolobus solfataricus
0.54
-
 3-phospho-D-glycerate 70°C, pH 6.5 Saccharolobus solfataricus
5.6
-
 3-phospho-D-glyceroyl phosphate 70°C, pH 6.5 Saccharolobus solfataricus
9.7
-
 ATP 70°C, pH 6.5 Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P50317
-
-
Saccharolobus solfataricus P2 P50317
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Reaction

Reaction Comment Organism Reaction ID
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate an ordered mechanism is assumed where 3-phospho-D-glycerate and 3-phospho-D-glyceroyl phosphate bind before ADP and ATP, respectively Saccharolobus solfataricus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-phospho-D-glycerate
-
 Saccharolobus solfataricus ADP + 3-phospho-D-glyceroyl phosphate
-
 r
ATP + 3-phospho-D-glycerate
-
 Saccharolobus solfataricus P2 ADP + 3-phospho-D-glyceroyl phosphate
-
 r

Synonyms

Synonyms Comment Organism
PGK
-
 Saccharolobus solfataricus
SSO0527 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14
-
 ADP 70°C, pH 6.5 Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function gluconeogenic pathway Saccharolobus solfataricus