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Literature summary for 2.7.2.3 extracted from

  • Troncoso-Ponce, M.A.; Rivoal, J.; Venegas-Caleron, M.; Dorion, S.; Sanchez, R.; Cejudo, F.J.; Garces, R.; Martinez-Force, E.
    Molecular cloning and biochemical characterization of three phosphoglycerate kinase isoforms from developing sunflower (Helianthus annuus L.) seeds (2012), Phytochemistry, 79, 27-38.
    View publication on PubMed
Search for more literature for 2.7.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Helianthus annuus

Inhibitors

Inhibitors Comment Organism Structure
ADP 0.1 mM, 63.1% activity; 0.1 mM, 85% activity; 0.1 mM, 86% activity; 1 mM, 31% activity; 1 mM, 32% activity Helianthus annuus
AMP 0.625 mM, 95% activity; 0.625 mM, 98% activity; 10 mM, 40% activity; 10 mM, 44% activity; 10 mM, 50% activity Helianthus annuus
Glycerol 2-phosphate 10 mM, 97% activity Helianthus annuus
glycerol 3-phosphate 10 mM, 92% activity; 10 mM, 95% activity; 10 mM, 97% activity Helianthus annuus
UDP 10 mM, 94.3% activity; 10 mM, 97% activity Helianthus annuus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.215
-
 ATP pH 8.2, 25°C Helianthus annuus
0.227
-
 ATP pH 7.6, 25°C Helianthus annuus
0.243
-
 ATP pH 7, 25°C Helianthus annuus
0.3
-
 ATP pH 7, 25°C Helianthus annuus
0.356
-
 ATP pH 8.2, 25°C Helianthus annuus
0.357
-
 ATP pH 7, 25°C Helianthus annuus
0.367
-
 ATP pH 7.6, 25°C Helianthus annuus
0.404
-
 ATP pH 8.2, 25°C Helianthus annuus
0.406
-
 ATP pH 7.6, 25°C Helianthus annuus
0.589
-
 3-phospho-D-glycerate pH 8.2, 25°C Helianthus annuus
0.601
-
 3-phospho-D-glycerate pH 7, 25°C Helianthus annuus
0.683
-
 3-phospho-D-glycerate pH 8.2, 25°C Helianthus annuus
0.707
-
 3-phospho-D-glycerate pH 7, 25°C Helianthus annuus
0.711
-
 3-phospho-D-glycerate pH 7.6, 25°C Helianthus annuus
0.719
-
 3-phospho-D-glycerate pH 7.6, 25°C Helianthus annuus
1.635
-
 3-phospho-D-glycerate pH 8.2, 25°C Helianthus annuus
1.787
-
 3-phospho-D-glycerate pH 7.6, 25°C Helianthus annuus
1.948
-
 3-phospho-D-glycerate pH 7, 25°C Helianthus annuus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Helianthus annuus 5829
-
plastid
-
 Helianthus annuus 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42300
-
 calculated from cDNA Helianthus annuus
42400
-
 calculated from cDNA Helianthus annuus
45000
-
 SDS-PAGE, recombinant protein Helianthus annuus

Organism

Organism UniProt Comment Textmining
Helianthus annuus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Helianthus annuus

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Helianthus annuus
-
cotyledon high expression Helianthus annuus
-
leaf
-
 Helianthus annuus
-
leaf high expression Helianthus annuus
-
root
-
 Helianthus annuus
-
stem
-
 Helianthus annuus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-phospho-D-glycerate
-
 Helianthus annuus ADP + 3-phospho-D-glyceroyl phosphate
-
 ?

Synonyms

Synonyms Comment Organism
HacPGK1
-
 Helianthus annuus
HacPGK2
-
 Helianthus annuus
HapPGK
-
 Helianthus annuus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Helianthus annuus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 80% activity at 65°C, then rapidly decreasing between 65-80°C Helianthus annuus
65
-
 enzymatic activity is completely lost at temperatures over 65°C Helianthus annuus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Helianthus annuus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.75 optimal pH range Helianthus annuus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9.2 75% activity retained at pH 9.2, a sharp reduction of activity below pH 7 Helianthus annuus
9.2
-
 75% activity retained at pH 9.2 Helianthus annuus

Expression

Organism Comment Expression
Helianthus annuus HacPGK1 shows the highest level of expression during seed development, especially at the initial stages, 12 days after flowering (DAF), and accounts for most (approximately 89%) of the combined expression of the three genes. As seed development progresses, the level of expression for this gene decreases up
Helianthus annuus highest expression of HapPGK in photosynthetically active tissues, cotyledons and leaves up