Crystallization (Comment) | Organism |
---|---|
crystals of the 3PG-HsPGK binary complex in an open conformation (HsPGK-3PG-open) are grown, and ADP and the nonhydrolyzable ATP analogue, AMP-PCP, are introduced, by soaking, to investigate the effect of their binding on the open conformation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00558 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate | small angle X-ray scattering (SAXS) data on complexes of human PGK (HsPGK) in solution. In combination with deformable elastic network (DEN) refinement a fully open conformation of the apoenzyme is defined, and details are revealed of the relative time spent by the enzyme in the open and closed conformations during catalytic turnover. Together with the crystal structures it is demonstrated that the enzyme strongly favors the open conformation | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + 3-phospho-D-glyceroyl phosphate | - |
Homo sapiens | ATP + 3-phospho-D-glycerate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HsPGK | - |
Homo sapiens |
phosphoglycerate kinase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |