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Literature summary for 2.7.2.3 extracted from

  • Pey, A.L.; Mesa-Torres, N.; Chiarelli, L.R.; Valentini, G.
    Structural and energetic basis of protein kinetic destabilization in human phosphoglycerate kinase 1 deficiency (2013), Biochemistry, 52, 1160-1170.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E252A specific activity (micromol/min/mg): 220 (wild-type: 420), Tm: 52.1°C, t1/2 (37°C): 7 months. Wild-type: Tm 52.8°C, t1/2(37°C) 3 years Homo sapiens
I47N specific activity (micromol/min/mg): 90 (wild-type: 420), Tm: 44.6°C, t1/2 (37°C): 27 min. Wild-type: Tm 52.8°C, t1/2(37°C) 3 years Homo sapiens
L89P specific activity (micromol/min/mg): 260 (wild-type: 420), Tm: 43°C, t1/2 (37°C): 13 min. Wild-type: Tm 52.8°C, t1/2(37°C) 3 years Homo sapiens
T378P specific activity (micromol/min/mg): 65 (wild-type: 420), Tm: 49.8°C, t1/2 (37°C): 3.9 days. Wild-type: Tm 52.8°C, t1/2(37°C) 3 years Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ion-exchange chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
65
-
mutant T378P, pH 7.4, 25°C Homo sapiens
90
-
mutant I47N, pH 7.4, 25°C Homo sapiens
220
-
mutant E252A, pH 7.4, 25°C Homo sapiens
260
-
mutant L89P, pH 7.4, 25°C Homo sapiens
420
-
wild-type, pH 7.4, 25°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-phospho-D-glycerate
-
Homo sapiens ADP + 3-phospho-D-glyceroyl phosphate
-
?

Synonyms

Synonyms Comment Organism
PGK1
-
Homo sapiens
phosphoglycerate kinase 1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52.8
-
Tm (wild-type): 52.8°C, t1/2 (wild-type, 37°C): 3 years Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens