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Literature summary for 2.7.2.3 extracted from

  • Mizuno, Y.; Ohshima, M.; Yao, Y.; Shibasaki, R.; Takahashi, R.; Tokuyama, T.
    Purification and comparison of phosphoglycerate kinases from nitrifying bacteria (1998), J. Ferment. Bioeng., 86, 346-350.
No PubMed abstract available
Search for more literature for 2.7.2.3

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate strong Nitrosomonas europaea
p-chloromercuribenzoate strong Nitrosomonas sp. K1
SDS strong Nitrosomonas europaea
SDS strong Nitrosomonas sp. K1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43400
-
 1 * 43400 Rhodopseudomonas palustris
43700
-
 1 * 43700 Nitrosomonas sp. K1
44300
-
 1 * 44300 Nitrosomonas sp. TNO632
44600
-
 1 * 44600 Nitrosomonas europaea
46100
-
 1 * 46100 Nitrobacter winogradskyi

Organism

Organism UniProt Comment Textmining
Nitrobacter winogradskyi
-
 IFO 14297
-
Nitrosomonas europaea
-
 ATCC 25978T
-
Nitrosomonas sp. K1
-
-
-
Nitrosomonas sp. TNO632
-
-
-
Rhodopseudomonas palustris
-
 strain JCM2524
-
Rhodopseudomonas palustris JCM 2524
-
 strain JCM2524
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Rhodopseudomonas palustris
to homogeneity Nitrosomonas europaea
to homogeneity Nitrobacter winogradskyi
to homogeneity Nitrosomonas sp. TNO632
to homogeneity Nitrosomonas sp. K1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-phospho-D-glycerate
-
 Rhodopseudomonas palustris ADP + 1,3-diphosphoglycerate
-
 r
ATP + 3-phospho-D-glycerate
-
 Nitrobacter winogradskyi ADP + 1,3-diphosphoglycerate
-
 r
ATP + 3-phospho-D-glycerate
-
 Nitrosomonas sp. TNO632 ADP + 1,3-diphosphoglycerate
-
 r
ATP + 3-phospho-D-glycerate specific for ATP Nitrosomonas europaea ADP + 1,3-diphosphoglycerate
-
 r
ATP + 3-phospho-D-glycerate specific for ATP Nitrosomonas sp. K1 ADP + 1,3-diphosphoglycerate
-
 r
ATP + 3-phospho-D-glycerate
-
 Rhodopseudomonas palustris JCM 2524 ADP + 1,3-diphosphoglycerate
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 44600 Nitrosomonas europaea
monomer 1 * 44300 Nitrosomonas sp. TNO632
monomer 1 * 46100 Nitrobacter winogradskyi
monomer 1 * 43700 Nitrosomonas sp. K1
monomer 1 * 43400 Rhodopseudomonas palustris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Nitrosomonas sp. K1
50
-
-
 Nitrosomonas europaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Nitrosomonas europaea
8 8.5
-
 Nitrosomonas sp. K1

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 stable at 4°C for 48 h Rhodopseudomonas palustris
7 9 stable at 4°C for 48 h Nitrosomonas europaea
7 9 stable at 4°C for 48 h Nitrobacter winogradskyi
7 9 stable at 4°C for 48 h Nitrosomonas sp. TNO632
7 9 stable at 4°C for 48 h Nitrosomonas sp. K1

Cofactor

Cofactor Comment Organism Structure
ADP
-
 Rhodopseudomonas palustris
ADP
-
 Nitrosomonas europaea
ADP
-
 Nitrobacter winogradskyi
ADP
-
 Nitrosomonas sp. TNO632
ADP
-
 Nitrosomonas sp. K1
ATP required as phosphate donor Rhodopseudomonas palustris
ATP required as phosphate donor Nitrosomonas europaea
ATP required as phosphate donor Nitrobacter winogradskyi
ATP required as phosphate donor Nitrosomonas sp. TNO632
ATP required as phosphate donor Nitrosomonas sp. K1