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Literature summary for 2.7.2.3 extracted from
- 3-Phosphoglycerate kinase from rat tissues. Further characterization and developmental studies (1974), Biochemistry, 13, 444-449.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | 0.2 | ATP | heart and skeletal muscle enzymes, pH 7.4, 25°C | Rattus norvegicus |  |
| 0.15 | 0.2 | ATP | ATP in form of MgATP2- | Rattus norvegicus | |
| 0.24 | - |
ATP | liver enzyme | Rattus norvegicus | |
| 1.4 | - |
3-phospho-D-glycerate | heart enzyme, pH 7.4, 25°C | Rattus norvegicus | |
| 1.65 | - |
3-phospho-D-glycerate | liver enzyme, pH 7.4, 25°C | Rattus norvegicus | |
| 1.66 | - |
3-phospho-D-glycerate | skeletal muscle enzyme, pH 7.4, 25°C | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
enzyme level in developing rat tissues | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cardiac muscle | - |
Rattus norvegicus | - |
| heart | of adult and newborn rats | Rattus norvegicus | - |
| heart ventricle | of adult and newborn rats | Rattus norvegicus | - |
| liver | of adult and newborn rats | Rattus norvegicus | - |
| skeletal muscle | of adult and newborn rats | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Rattus norvegicus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Rattus norvegicus | ADP + 3-phospho-D-glyceroyl phosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Rattus norvegicus | |
| ATP | Na2ATP2- | Rattus norvegicus | |
| ATP | required as phosphate donor | Rattus norvegicus | |
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