Any feedback?
Literature summary for 2.7.2.3 extracted from
- Phosphoglycerate kinase B from ram testis. Purification, characterisation and comparison with the muscle isoenzyme (1978), Eur. J. Biochem., 85, 89-95.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
ATP | pH 7.5, isozyme PGKA, in presence of 0.05 mM 1,3-bisphosphoglycerate | Ovis aries |  |
| 0.09 | - |
ATP | ATP in form of MgATP2- | Ovis aries | |
| 0.095 | - |
ATP | pH 7.5, isozyme PGKB, in presence of 0.05 mM 1,3-bisphosphoglycerate | Ovis aries | |
| 0.095 | - |
ATP | ATP in form of MgATP2- | Ovis aries | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
isozyme PGKA and PGKB, tryptophan content | Ovis aries |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
ram | - |
| Ovis aries | - |
testis-specific isozyme PGKB, isozyme PGKA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isozymes: PGKA and PGKB, from testis to homogeneity | Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | isozyme PGKA | Ovis aries | - |
| testis | testis-specific isozyme PGKB, isozyme PGKA | Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 700 | - |
purified isozyme PGKA | Ovis aries |
| 720 | - |
purified isozyme PGKB | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Ovis aries | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Ovis aries | ADP + 3-phospho-D-glyceroyl phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, SDS-PAGE | Ovis aries |
| monomer | 1 * 47000-47500, isozymes PGKA and PGKB, SDS-PAGE | Ovis aries |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal denaturation at pH 5.5 and pH 7.5 of the isoenzymes | Ovis aries |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Ovis aries |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Ovis aries | |
| ATP | true substrate is the magnesium complexes of ATP | Ovis aries | |
| ATP | required as phosphate donor | Ovis aries | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
