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Literature summary for 2.7.2.3 extracted from
- Isolation and characterization of the cytosolic and chloroplastic 3-phosphoglycerate kinase from spinach leaves (1990), Plant Physiol., 93, 40-47.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | biphasic kinetics | Spinacia oleracea |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
| cytosol | - |
Spinacia oleracea | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
gel filtration, cytosolic and chloroplastic isoenzyme | Spinacia oleracea |
| 40700 | - |
1 * 40700, cytosolic isozyme, SDS-PAGE | Spinacia oleracea |
| 41000 | - |
1 * 41000, chloroplastic isozyme, SDS-PAGE | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
2 isozymes, cytosolic and chloroplastic | - |
| Spinacia oleracea | - |
Sorte Noveto | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cytosolic, 160fold, and chloroplastic, 200fold, isozymes, to homogeneity | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 798 | - |
purified cytosolic isozyme | Spinacia oleracea |
| 800 | - |
purified chloroplastic isozyme | Spinacia oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, enzyme in the fractions from the phenyl-Sepharose column, stable for at least 2 months | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Spinacia oleracea | ADP + 1,3-diphosphoglycerate | - |
r |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40700, cytosolic isozyme, SDS-PAGE | Spinacia oleracea |
| monomer | 1 * 41000, chloroplastic isozyme, SDS-PAGE | Spinacia oleracea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Spinacia oleracea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
unstable above | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
both isozymes | Spinacia oleracea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Spinacia oleracea | |
| ATP | required as phosphate donor | Spinacia oleracea | |
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Release 2023.1 (January 2023)
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