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Literature summary for 2.7.2.3 extracted from
- Human phosphoglycerate kinase (1975), Methods Enzymol., 42C, 144-148.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystallization via precipitation with ammonium sulfate stepwise from 30-35% to 65% w/v within 24 h | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| freeze-drying inactivates | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | inhibition above 50 mM | Homo sapiens |  |
| Mn2+ | inhibition above 50 mM | Homo sapiens | |
| phosphate | phosphate buffer | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km for ATP at different concentrations | Homo sapiens | |
| 0.59 | - |
3-phospho-D-glycerate | pH 7.5, 25°C | Homo sapiens | |
| 1.1 | - |
3-phospho-D-glycerate | at 0.1 M | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| divalent cation | Mg2+ or Mn2+ | Homo sapiens | |
| Mg2+ | required | Homo sapiens | |
| Mg2+ | 5 mM Mg2+ or 10 mM Mn2+ required | Homo sapiens | |
| Mn2+ | Mn2+ or Mg2+ required | Homo sapiens | |
| Mn2+ | 5 mM Mg2+ or 10 mM Mn2+ required | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49600 | - |
sedimentation equilibrium method | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood | - |
Homo sapiens | - |
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 650 | 700 | purified enzyme, at 25°C | Homo sapiens |
| 1000 | - |
purified enzyme, at 35°C | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, with 10 mg/ml bovine serum albumin, stable for at least 1 week | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Homo sapiens | ADP + 1,3-diphosphoglycerate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
| 35 | - |
- |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 9 | - |
Homo sapiens |
| 7.5 | - |
assay at | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | enzyme in crude hemolysate is stable at 4°C for at least 1 week, inactive below pH 6.0 | Homo sapiens |
| 6.5 | 8 | crystallized enzyme, solved at a low concentration loses 10% activity per 24 h | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Homo sapiens | |
| ATP | required as phosphate donor | Homo sapiens | |
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Release 2023.1 (January 2023)
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