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Literature summary for 2.7.2.3 extracted from

  • Kuntz, G.W.K.; Krietsch, W.K.G.
    Phosphoglycerate kinase from spinach, blue-green algae, and yeast (1982), Methods Enzymol., 90, 110-114.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
Saccharomyces cerevisiae
5,5'-dithiobis(2-nitrobenzoic acid)
-
Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
ADP ADP in form of MgADP- Saccharomyces cerevisiae
0.27
-
ADP ADP in form of MgADP- Spinacia oleracea
0.3
-
ATP ATP in form of MgATP2- Spinacia oleracea
0.48
-
ATP ATP in form of MgATP2- Saccharomyces cerevisiae
0.48
-
ATP ATP in form of MgATP2- Spirulina geitleri
1.1
-
3-phospho-D-glycerate at 1.5-13 mM 3-phospho-D-glycerate concentration Spinacia oleracea
1.28
-
3-phospho-D-glycerate at 1.5-13 mM 3-phospho-D-glycerate concentration Saccharomyces cerevisiae
1.5
-
3-phospho-D-glycerate at 1.5-13 mM 3-phospho-D-glycerate concentration Spirulina geitleri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
x * 46000, SDS-PAGE Saccharomyces cerevisiae
46000
-
x * 46000, SDS-PAGE Spinacia oleracea
46000
-
x * 46000, SDS-PAGE Spirulina geitleri

Organism

Organism UniProt Comment Textmining
Arthrospira platensis
-
-
-
Saccharomyces cerevisiae
-
-
-
Spinacia oleracea
-
-
-
Spirulina geitleri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arthrospira platensis
106fold Spinacia oleracea
131fold Saccharomyces cerevisiae
36fold Spirulina geitleri

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
702
-
purified enzyme Spinacia oleracea
720
-
purified enzyme Spirulina geitleri
785
-
purified enzyme Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
as a precipitate in 3.3 M ammonium sulfate, stable for years Saccharomyces cerevisiae
as a precipitate in 3.3 M ammonium sulfate, stable for years Spinacia oleracea
as a precipitate in 3.3 M ammonium sulfate, stable for years Spirulina geitleri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + 3-phospho-D-glyceroyl phosphate
-
Saccharomyces cerevisiae ATP + 3-phospho-D-glycerate
-
r
ADP + 3-phospho-D-glyceroyl phosphate
-
Spinacia oleracea ATP + 3-phospho-D-glycerate
-
r
ATP + 3-phospho-D-glycerate
-
Arthrospira platensis ADP + 1,3-diphosphoglycerate
-
r
ATP + 3-phospho-D-glycerate high specificity for ATP Saccharomyces cerevisiae ADP + 1,3-diphosphoglycerate
-
r
ATP + 3-phospho-D-glycerate high specificity for ATP Spinacia oleracea ADP + 1,3-diphosphoglycerate
-
r
ATP + 3-phospho-D-glycerate high specificity for ATP Spirulina geitleri ADP + 1,3-diphosphoglycerate
-
r
ATP + 3-phospho-D-glycerate
-
Saccharomyces cerevisiae ADP + 3-phospho-D-glyceroyl phosphate
-
r
ATP + 3-phospho-D-glycerate
-
Spinacia oleracea ADP + 3-phospho-D-glyceroyl phosphate
-
r
ATP + 3-phospho-D-glycerate
-
Spirulina geitleri ADP + 3-phospho-D-glyceroyl phosphate
-
r
CTP + 3-phospho-D-glycerate no activity Saccharomyces cerevisiae CDP + 1,3-diphosphoglycerate
-
?
CTP + 3-phospho-D-glycerate no activity Spinacia oleracea CDP + 1,3-diphosphoglycerate
-
?
CTP + 3-phospho-D-glycerate no activity Spirulina geitleri CDP + 1,3-diphosphoglycerate
-
?
dATP + 3-phospho-D-glycerate
-
Saccharomyces cerevisiae dADP + 1,3-diphosphoglycerate
-
?
dATP + 3-phospho-D-glycerate low activity Spirulina geitleri dADP + 1,3-diphosphoglycerate
-
?
dGTP + 3-phospho-D-glycerate
-
Saccharomyces cerevisiae dGDP + 1,3-diphosphoglycerate
-
?
dGTP + 3-phospho-D-glycerate no activity Spirulina geitleri dGDP + 1,3-diphosphoglycerate
-
?
dITP + 3-phospho-D-glycerate
-
Saccharomyces cerevisiae dIDP + 1,3-diphosphoglycerate
-
?
dITP + 3-phospho-D-glycerate no activity Spirulina geitleri dIDP + 1,3-diphosphoglycerate
-
?
GTP + 3-phospho-D-glycerate
-
Saccharomyces cerevisiae GDP + 1,3-diphosphoglycerate
-
?
GTP + 3-phospho-D-glycerate very low activity Spirulina geitleri GDP + 1,3-diphosphoglycerate
-
?
GTP + 3-phospho-D-glycerate low activity Spinacia oleracea GDP + 1,3-diphosphoglycerate
-
?
ITP + 3-phospho-D-glycerate
-
Saccharomyces cerevisiae IDP + 1,3-diphosphoglycerate
-
?
ITP + 3-phospho-D-glycerate very low activity Spirulina geitleri IDP + 1,3-diphosphoglycerate
-
?
ITP + 3-phospho-D-glycerate low activity Spinacia oleracea IDP + 1,3-diphosphoglycerate
-
?
additional information no activity with dCTP, dUTP, dTTP Saccharomyces cerevisiae ?
-
?
additional information no activity with dCTP, dUTP, dTTP Spinacia oleracea ?
-
?
additional information no activity with dCTP, dUTP, dTTP Spirulina geitleri ?
-
?
UTP + 3-phospho-D-glycerate low activity Spinacia oleracea UDP + 1,3-diphosphoglycerate
-
?
UTP + 3-phospho-D-glycerate only traces of activity Saccharomyces cerevisiae UDP + 1,3-diphosphoglycerate
-
?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Saccharomyces cerevisiae
? x * 46000, SDS-PAGE Spinacia oleracea
? x * 46000, SDS-PAGE Spirulina geitleri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Saccharomyces cerevisiae
25
-
assay at Spinacia oleracea
25
-
assay at Spirulina geitleri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9.2
-
Saccharomyces cerevisiae
6.5 9.5
-
Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
ADP
-
Saccharomyces cerevisiae
ADP
-
Spinacia oleracea
ADP
-
Arthrospira platensis
ADP
-
Spirulina geitleri
ATP required as phosphate donor Saccharomyces cerevisiae
ATP required as phosphate donor Spinacia oleracea
ATP required as phosphate donor Arthrospira platensis
ATP required as phosphate donor Spirulina geitleri
dATP
-
Saccharomyces cerevisiae
dATP
-
Spirulina geitleri
dGTP
-
Saccharomyces cerevisiae
dITP
-
Saccharomyces cerevisiae
GTP
-
Saccharomyces cerevisiae
GTP
-
Spinacia oleracea
GTP
-
Spirulina geitleri
ITP
-
Saccharomyces cerevisiae
ITP
-
Spinacia oleracea
ITP
-
Spirulina geitleri
UTP
-
Saccharomyces cerevisiae
UTP
-
Spinacia oleracea

pI Value

Organism Comment pI Value Maximum pI Value
Spinacia oleracea
-
4.4 4.3
Saccharomyces cerevisiae thin-layer isoelectric focusing 4.4 4.3
Spirulina geitleri
-
-
4.8
Saccharomyces cerevisiae thin-layer isoelectric focusing
-
4.8
Saccharomyces cerevisiae thin-layer isoelectric focusing
-
5.3