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Literature summary for 2.7.2.2 extracted from
- Kinetic characterization of arginine deiminase and carbamate kinase from Streptococcus pyogenes M49 (2013), Protein Expr. Purif., 91, 61-68.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene arcC, genetic organization in the arc operon, sequence comparisons, recombinant expression in Escherichia coli strain DH5alpha | Streptococcus pyogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Streptococcus pyogenes |  |
| L-arginine | slight inhibition | Streptococcus pyogenes | |
| L-citrulline | - |
Streptococcus pyogenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
Carbamoyl phosphate | recombinant enzyme, pH 6.5, 37°C | Streptococcus pyogenes | |
| 0.72 | - |
ADP | recombinant enzyme, pH 6.5, 37°C | Streptococcus pyogenes | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Streptococcus pyogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34200 | - |
x * 34200, SDS-PAGE | Streptococcus pyogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + carbamoyl phosphate | Streptococcus pyogenes | - |
ATP + NH3 + CO2 | - |
r | |
| ATP + NH3 + CO2 | Streptococcus pyogenes | - |
ADP + carbamoyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pyogenes | A0A0H3BY05 | serotype M49, strain 591, gene arcC | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain DH5alpha by affinity chromatography on StrepTactin resin | Streptococcus pyogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + carbamoyl phosphate | - |
Streptococcus pyogenes | ATP + NH3 + CO2 | - |
r | |
| ATP + NH3 + CO2 | - |
Streptococcus pyogenes | ADP + carbamoyl phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34200, SDS-PAGE | Streptococcus pyogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ArcC | - |
Streptococcus pyogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Streptococcus pyogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Streptococcus pyogenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Streptococcus pyogenes | |
| ATP | - |
Streptococcus pyogenes | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is part of the the arginine deiminase system, overview | Streptococcus pyogenes |
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Release 2023.1 (January 2023)
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