Literature summary for 2.7.2.2 extracted from

Galkin, A.; Kulakova, L.; Wu, R.; Nash, T.E.; Dunaway-Mariano, D.; Herzberg, O.
X-ray structure and characterization of carbamate kinase from the human parasite Giardia lamblia (2010), Acta Crystallogr. Sect. F, 66, 386-390.

Search for more literature for 2.7.2.2

Crystallization (Commentary)

Crystallization (Comment)	Organism
the three-dimensional structure of carbamate kinase from the human parasite Giardia lamblia is determined at 3 A resolution. The crystals belong to the monoclinic space group P21, with unit-cell parameters a = 69.77, b = 85.41, c = 102.1 A, beta = 106.8°	Giardia intestinalis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.035	-	ATP	pH 7.5, 25°C	Giardia intestinalis
0.07	-	ADP	pH 7.5, 25°C	Giardia intestinalis
0.085	-	Carbamoyl phosphate	pH 7.5, 25°C	Giardia intestinalis
43	-	Carbamate	pH 7.5, 25°C	Giardia intestinalis

Organism

Organism	UniProt	Comment	Textmining
Giardia intestinalis	A8BB85	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + carbamoyl phosphate	-	Giardia intestinalis	ATP + NH3 + CO2	-	?
ATP + carbamate	-	Giardia intestinalis	ADP + carbamoyl phosphate	-	?

Synonyms

Synonyms	Comment	Organism
carbamate kinase	-	Giardia intestinalis
GlcK	-	Giardia intestinalis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
43	-	Carbamate	pH 7.5, 25°C	Giardia intestinalis
319	-	ADP	pH 7.5, 25°C	Giardia intestinalis
319	-	Carbamoyl phosphate	pH 7.5, 25°C	Giardia intestinalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.001	-	Carbamate	pH 7.5, 25°C	Giardia intestinalis
70000	-	ADP	pH 7.5, 25°C	Giardia intestinalis
85000	-	Carbamoyl phosphate	pH 7.5, 25°C	Giardia intestinalis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)