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Literature summary for 2.7.2.2 extracted from

  • Uriarte, M.; Marina, A.; Ramon-Maiques, S.; Fita, I.; Rubio, V.
    The carbamoyl-phosphate synthetase of Pyrococcus furiosus is enzymologically and structurally a carbamate kinase (1999), J. Biol. Chem., 274, 16295-16303.
    View publication on PubMed
Search for more literature for 2.7.2.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pyrococcus furiosus
expression in Escherichia coli Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of the pyrococcal enzyme grown in the absence or presence of MgATP (Fig. 7) diffracted with a conventional X-ray source to at least 2.6 and 2.0 Å resolution, respectively Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 34000, SDS-PAGE Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + carbamoyl phosphate Pyrococcus furiosus carbamate kinase with an anabolic role. The extreme living conditions of the pyrococci may render unnecessary the enzymatic synthesis of carbamate by carbamoyl-phosphate synthetase with the associated expenditure of an extra ATP molecule, as a prelude to making carbamoyl phosphate, explaining the use of carbamate kinase for carbamoyl phosphate synthesis in this organisms ATP + NH3 + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-
Pyrococcus furiosus P95474
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + carbamoyl phosphate
-
 Pyrococcus furiosus ATP + NH3 + CO2
-
 ?
ADP + carbamoyl phosphate carbamate kinase with an anabolic role. The extreme living conditions of the pyrococci may render unnecessary the enzymatic synthesis of carbamate by carbamoyl-phosphate synthetase with the associated expenditure of an extra ATP molecule, as a prelude to making carbamoyl phosphate, explaining the use of carbamate kinase for carbamoyl phosphate synthesis in this organisms Pyrococcus furiosus ATP + NH3 + CO2
-
 ?
ATP + NH3 + CO2 carbamoyl phosphate synthetase is a carbamate synthase Pyrococcus furiosus ADP + carbamoyl phosphate
-
 r

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
CK-like CPS
-
 Pyrococcus furiosus