Cloned (Comment) | Organism |
---|---|
gene PRO1, cloning of mutants in Escherichia coli strain DH5alpha | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D156A | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
additional information | construction of a gene PRO1 disruption mutant, phenotype, detailed overview. Overexpression of wild-type enzyme partially suppresses the phenotype of the DELTApro1 strain, which is deficient in ribophagy | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate | Saccharomyces cerevisiae | - |
ADP + L-glutamate 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P32264 | gene PRO1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate | - |
Saccharomyces cerevisiae | ADP + L-glutamate 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gamma-glutamyl kinase | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | the DELTApro1 cells are more sensitive to various stresses than wild-type cells. Yeast cells with gene PRO1 deletion or expressing inactive enzyme display a defect for ribophagy but not for nonselective autophagy. Yeast strains deficient ribophagy are sensitive to nitrogen starvation | Saccharomyces cerevisiae |
metabolism | gamma-glutamyl kinase is a key enzyme in the Saccharomyces cerevisiae proline biosynthesis pathway | Saccharomyces cerevisiae |
additional information | conserved residue Asp156 plays an important role in the catalytic activity | Saccharomyces cerevisiae |
physiological function | the enzyme has an alternative function independent of proline biosynthesis. Gene PRO1 genetically interacts with UBP3, which encodes ubiquitin-specific protease, and is required for selective autophagy of ribosomes (ribophagy). The enzyme activity is indispensable for ribophagy, ribophagy is important for cell survival during nitrogen starvation | Saccharomyces cerevisiae |