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Literature summary for 2.7.2.11 extracted from

  • Tatehashi, Y.; Watanabe, D.; Takagi, H.
    gamma-Glutamyl kinase is involved in selective autophagy of ribosomes in Saccharomyces cerevisiae (2016), FEBS Lett., 590, 2906-2914.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene PRO1, cloning of mutants in Escherichia coli strain DH5alpha Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D156A site-directed mutagenesis, inactive mutant Saccharomyces cerevisiae
additional information construction of a gene PRO1 disruption mutant, phenotype, detailed overview. Overexpression of wild-type enzyme partially suppresses the phenotype of the DELTApro1 strain, which is deficient in ribophagy Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate Saccharomyces cerevisiae
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ADP + L-glutamate 5-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P32264 gene PRO1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate
-
Saccharomyces cerevisiae ADP + L-glutamate 5-phosphate
-
?

Synonyms

Synonyms Comment Organism
gamma-glutamyl kinase
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Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction the DELTApro1 cells are more sensitive to various stresses than wild-type cells. Yeast cells with gene PRO1 deletion or expressing inactive enzyme display a defect for ribophagy but not for nonselective autophagy. Yeast strains deficient ribophagy are sensitive to nitrogen starvation Saccharomyces cerevisiae
metabolism gamma-glutamyl kinase is a key enzyme in the Saccharomyces cerevisiae proline biosynthesis pathway Saccharomyces cerevisiae
additional information conserved residue Asp156 plays an important role in the catalytic activity Saccharomyces cerevisiae
physiological function the enzyme has an alternative function independent of proline biosynthesis. Gene PRO1 genetically interacts with UBP3, which encodes ubiquitin-specific protease, and is required for selective autophagy of ribosomes (ribophagy). The enzyme activity is indispensable for ribophagy, ribophagy is important for cell survival during nitrogen starvation Saccharomyces cerevisiae