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Literature summary for 2.7.2.11 extracted from

  • Miyoshi, K.; Matsuura, H.; Kumon, T.; Matsuda, K.; Yoshikawa, T.; Bamba, T.; Harada, K.; Hirata, K.
    Isolation and partial characterization of the Chlamydomonas reinhardtii gamma-glutamyl kinase (2011), J. Biosci. Bioeng., 112, 75-78.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
L-proline results indicate that the sensitivity of Chlamydomonas glutamte kinase to feedback inhibition by L-proline is much lower than that of similar microbial glutamte kinase Chlamydomonas reinhardtii

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast computer predictions suggest that a chloroplast transit peptide exists at the N-terminal region Chlamydomonas reinhardtii 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
SDS-PAGE, recombinant protein Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate
-
Chlamydomonas reinhardtii ADP + L-glutamate 5-phosphate
-
?

Synonyms

Synonyms Comment Organism
gamma-glutamyl kinase
-
Chlamydomonas reinhardtii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Chlamydomonas reinhardtii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
90
-
pH 7.0, 37°C Chlamydomonas reinhardtii L-proline

General Information

General Information Comment Organism
physiological function Chlamydomonas reinhardtii gamma-glutamte kinase is functional as demonstrated by successful complementation of Escherichia coli auxotroph JW0232 (strain in which the proB gene has been knocked-out) Chlamydomonas reinhardtii