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Literature summary for 2.7.2.10 extracted from

  • Reeves, R.E.; South, D.J.
    Phosphoglycerate kinase (GTP). An enzyme from Entamoeba histolytica selective for guanine nucleotides (1974), Biochem. Biophys. Res. Commun., 58, 1053-1057.
    View publication on PubMed
Search for more literature for 2.7.2.10

General Stability

General Stability Organism
upon concentration by vacuum dialysis against 20 mM imidazole buffer, pH 7, a loss of about 40% of activity occurs Entamoeba histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 GTP pH 7, 30°C Entamoeba histolytica
0.3
-
 GDP pH 7, 30°C Entamoeba histolytica
0.4
-
 3-phospho-D-glycerate pH 7, 30°C Entamoeba histolytica
0.7
-
 IDP pH 7, 30°C Entamoeba histolytica
0.8
-
 ITP pH 7, 30°C Entamoeba histolytica
1.2
-
 ATP pH 7, 30°C Entamoeba histolytica
10
-
 ADP pH 7, 30°C Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP + 3-phospho-D-glyceroyl 1-phosphate Entamoeba histolytica production of GTP GTP + 3-phospho-D-glycerate
-
 r

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Entamoeba histolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
52
-
 GDP + 3-phospho-D-glyceroyl phosphate Entamoeba histolytica

Storage Stability

Storage Stability Organism
4°C, concentrated enzyme solution, 10% loss of activity per week Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-phospho-D-glycerate in glycolytic direction selectivity for GDP over ADP is 150-fold, selectivity for GTP over ATP is about 50fold Entamoeba histolytica ADP + 3-phospho-D-glyceroyl 1-phosphate
-
 r
GDP + 3-phospho-D-glyceroyl 1-phosphate production of GTP Entamoeba histolytica GTP + 3-phospho-D-glycerate
-
 r
GTP + 3-phospho-D-glycerate in glycolytic direction selectivity for GDP over ADP is 150-fold, selectivity for GTP over ATP is about 50fold Entamoeba histolytica GDP + 3-phospho-D-glyceroyl 1-phosphate
-
 r
GTP + 3-phospho-D-glycerate
-
 Entamoeba histolytica GDP + 3-phospho-D-glyceroyl phosphate
-
 ?
ITP + 3-phospho-D-glycerate in glycolytic direction 40% of the activity with GDP, 30% of the activity with GTP Entamoeba histolytica IDP + 3-phospho-D-glyceroyl 1-phosphate
-
 r