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show all sequences of 2.7.2.1

Cold adaptation: structural and functional characterizations of psychrophilic and mesophilic acetate kinase

Tang, M.A.; Motoshima, H.; Watanabe, K.; Protein J. 33, 313-322 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene ackA, recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Saccharomyces cerevisiae strain YPH499
Shewanella sp.
gene ackA, recombinant His-tagged enzyme overexpression in Escherichia coli strain BL21(DE3), subcloning in Saccharomyces cerevisiae strain YPH499
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics, thermodynamics
Escherichia coli
additional information
-
additional information
Michaelis-Menten kinetics, thermodynamics
Shewanella sp.
0.47
-
ADP
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
0.48
-
ATP
pH 7.5, 15C, recombinant enzyme; pH 7.5, 20C, recombinant enzyme
Shewanella sp.
0.49
-
ADP
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
0.49
-
ATP
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
0.5
-
acetyl phosphate
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
0.5
-
ADP
pH 7.5, 10C, recombinant enzyme; pH 7.5, 15C, recombinant enzyme
Shewanella sp.
0.5
-
ATP
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
0.53
-
acetyl phosphate
pH 7.5, 10C, recombinant enzyme; pH 7.5, 15C, recombinant enzyme; pH 7.5, 20C, recombinant enzyme
Shewanella sp.
0.57
-
ATP
pH 7.5, 20C, recombinant enzyme
Escherichia coli
0.59
-
acetyl phosphate
pH 7.5, 25C, recombinant enzyme
Escherichia coli
0.59
-
ATP
pH 7.5, 25C, recombinant enzyme
Escherichia coli
0.61
-
ADP
pH 7.5, 25C, recombinant enzyme
Escherichia coli
0.62
-
ADP
pH 7.5, 20C, recombinant enzyme
Escherichia coli
0.64
-
ADP
pH 7.5, 15C, recombinant enzyme
Escherichia coli
0.64
-
ATP
pH 7.5, 15C, recombinant enzyme
Escherichia coli
0.65
-
ATP
pH 7.5, 10C, recombinant enzyme
Escherichia coli
0.66
-
ADP
pH 7.5, 10C, recombinant enzyme
Escherichia coli
0.67
-
acetyl phosphate
pH 7.5, 20C, recombinant enzyme
Escherichia coli
0.69
-
acetyl phosphate
pH 7.5, 10C, recombinant enzyme
Escherichia coli
0.71
-
acetyl phosphate
pH 7.5, 15C, recombinant enzyme
Escherichia coli
1.61
-
acetate
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
1.63
-
acetate
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
1.71
-
acetate
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
1.77
-
acetate
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
29.3
-
acetate
pH 7.5, 25C, recombinant enzyme
Escherichia coli
31.32
-
acetate
pH 7.5, 20C, recombinant enzyme
Escherichia coli
32.26
-
acetate
pH 7.5, 15C, recombinant enzyme
Escherichia coli
36.02
-
acetate
pH 7.5, 10C, recombinant enzyme
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
the enzyme requires Mn2+ or Mg2+
Escherichia coli
Mg2+
required
Shewanella sp.
Mn2+
the enzyme requires Mn2+ or Mg2+
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
-
Escherichia coli
43000
-
-
Shewanella sp.
43300
-
-
Escherichia coli
43700
-
-
Shewanella sp.
86000
-
gel filtration, recombinant enzyme
Escherichia coli
86000
-
gel filtration, recombinant enzyme
Shewanella sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + acetate
Escherichia coli
-
ADP + acetyl phosphate
-
-
r
ATP + acetate
Shewanella sp.
-
ADP + acetyl phosphate
-
-
r
ATP + acetate
Shewanella sp. AS-11
-
ADP + acetyl phosphate
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Shewanella sp.
E3W769
gene ackA
-
Shewanella sp. AS-11
E3W769
gene ackA
-
Escherichia coli
P0A6A3
gene ackA
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by affinity chromatography and anion exchange chromatography
Escherichia coli
recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by affinity chromatography and anion exchange chromatography
Shewanella sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl phosphate + hydroxylamine
-
739591
Escherichia coli
acetyl hydroxamate + phosphate
-
-
-
?
acetyl phosphate + hydroxylamine
-
739591
Shewanella sp.
acetyl hydroxamate + phosphate
-
-
-
?
acetyl phosphate + hydroxylamine
-
739591
Shewanella sp. AS-11
acetyl hydroxamate + phosphate
-
-
-
?
ATP + acetate
-
739591
Escherichia coli
ADP + acetyl phosphate
-
-
-
r
ATP + acetate
-
739591
Shewanella sp.
ADP + acetyl phosphate
-
-
-
r
ATP + acetate
the equilibrium lies far to the left side
739591
Escherichia coli
ADP + acetyl phosphate
-
-
-
r
ATP + acetate
-
739591
Shewanella sp. AS-11
ADP + acetyl phosphate
-
-
-
r
Subunits
Subunits
Commentary
Organism
homodimer
2 * 42000, recombinant enzyme, SDS-PAGE, 2 * 43300, about, sequence calculation
Escherichia coli
homodimer
2 * 43000, recombinant enzyme, SDS-PAGE, 2 * 43700, about, sequence calculation
Shewanella sp.
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
about
Shewanella sp.
70
-
about
Escherichia coli
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
20
80
purified recombinant His-tagged enzyme, pH 7.5, 50% of maximal activity remaining after 15 min at 55C for the free enzyme, at 65C for the acetate-bound enzyme, and at 70C for the ATP-bound enzyme. Free enzyme is completely stable up to 40C, inactivation of all enzymes at 80C
Escherichia coli
20
80
purified recombinant His-tagged enzyme, pH 7.5, the enzyme is completely stable up to 30C, half-life is about 40-50C, inactivation at 80C
Shewanella sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
195
-
acetate
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
205
-
ATP
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
235
-
ATP
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
245
-
acetate
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
300
-
acetate
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
305
-
ATP
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
350
-
ATP
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
385
-
acetate
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
560
-
acetate
pH 7.5, 10C, recombinant enzyme
Escherichia coli
565
-
ATP
pH 7.5, 10C, recombinant enzyme
Escherichia coli
745
-
ATP
pH 7.5, 15C, recombinant enzyme
Escherichia coli
760
-
acetate
pH 7.5, 15C, recombinant enzyme
Escherichia coli
875
-
acetyl phosphate
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
884
-
ADP
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
890
-
acetyl phosphate
pH 7.5, 10C, recombinant enzyme
Escherichia coli
892
-
ADP
pH 7.5, 10C, recombinant enzyme
Escherichia coli
1130
-
ATP
pH 7.5, 20C, recombinant enzyme
Escherichia coli
1165
-
acetate
pH 7.5, 20C, recombinant enzyme
Escherichia coli
1340
-
acetyl phosphate
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
1350
-
ADP
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
1420
-
acetate
pH 7.5, 25C, recombinant enzyme
Escherichia coli
1500
-
ATP
pH 7.5, 25C, recombinant enzyme
Escherichia coli
1550
-
acetyl phosphate
pH 7.5, 15C, recombinant enzyme
Escherichia coli
1565
-
ADP
pH 7.5, 15C, recombinant enzyme
Escherichia coli
1700
-
acetyl phosphate
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
1700
-
ADP
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
2100
-
acetyl phosphate
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
2135
-
ADP
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
2150
-
acetyl phosphate
pH 7.5, 20C, recombinant enzyme
Escherichia coli
2178
-
ADP
pH 7.5, 20C, recombinant enzyme
Escherichia coli
3515
-
ADP
pH 7.5, 25C, recombinant enzyme
Escherichia coli
3600
-
acetyl phosphate
pH 7.5, 25C, recombinant enzyme
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Escherichia coli
7.5
-
assay at
Shewanella sp.
Cofactor
Cofactor
Commentary
Organism
Structure
ADP
-
Escherichia coli
ADP
-
Shewanella sp.
ATP
-
Escherichia coli
ATP
-
Shewanella sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ackA, recombinant His-tagged enzyme overexpression in Escherichia coli strain BL21(DE3), subcloning in Saccharomyces cerevisiae strain YPH499
Escherichia coli
gene ackA, recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Saccharomyces cerevisiae strain YPH499
Shewanella sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ADP
-
Escherichia coli
ADP
-
Shewanella sp.
ATP
-
Escherichia coli
ATP
-
Shewanella sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics, thermodynamics
Escherichia coli
additional information
-
additional information
Michaelis-Menten kinetics, thermodynamics
Shewanella sp.
0.47
-
ADP
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
0.48
-
ATP
pH 7.5, 15C, recombinant enzyme; pH 7.5, 20C, recombinant enzyme
Shewanella sp.
0.49
-
ADP
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
0.49
-
ATP
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
0.5
-
acetyl phosphate
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
0.5
-
ADP
pH 7.5, 10C, recombinant enzyme; pH 7.5, 15C, recombinant enzyme
Shewanella sp.
0.5
-
ATP
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
0.53
-
acetyl phosphate
pH 7.5, 10C, recombinant enzyme; pH 7.5, 15C, recombinant enzyme; pH 7.5, 20C, recombinant enzyme
Shewanella sp.
0.57
-
ATP
pH 7.5, 20C, recombinant enzyme
Escherichia coli
0.59
-
acetyl phosphate
pH 7.5, 25C, recombinant enzyme
Escherichia coli
0.59
-
ATP
pH 7.5, 25C, recombinant enzyme
Escherichia coli
0.61
-
ADP
pH 7.5, 25C, recombinant enzyme
Escherichia coli
0.62
-
ADP
pH 7.5, 20C, recombinant enzyme
Escherichia coli
0.64
-
ADP
pH 7.5, 15C, recombinant enzyme
Escherichia coli
0.64
-
ATP
pH 7.5, 15C, recombinant enzyme
Escherichia coli
0.65
-
ATP
pH 7.5, 10C, recombinant enzyme
Escherichia coli
0.66
-
ADP
pH 7.5, 10C, recombinant enzyme
Escherichia coli
0.67
-
acetyl phosphate
pH 7.5, 20C, recombinant enzyme
Escherichia coli
0.69
-
acetyl phosphate
pH 7.5, 10C, recombinant enzyme
Escherichia coli
0.71
-
acetyl phosphate
pH 7.5, 15C, recombinant enzyme
Escherichia coli
1.61
-
acetate
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
1.63
-
acetate
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
1.71
-
acetate
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
1.77
-
acetate
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
29.3
-
acetate
pH 7.5, 25C, recombinant enzyme
Escherichia coli
31.32
-
acetate
pH 7.5, 20C, recombinant enzyme
Escherichia coli
32.26
-
acetate
pH 7.5, 15C, recombinant enzyme
Escherichia coli
36.02
-
acetate
pH 7.5, 10C, recombinant enzyme
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
the enzyme requires Mn2+ or Mg2+
Escherichia coli
Mg2+
required
Shewanella sp.
Mn2+
the enzyme requires Mn2+ or Mg2+
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
-
Escherichia coli
43000
-
-
Shewanella sp.
43300
-
-
Escherichia coli
43700
-
-
Shewanella sp.
86000
-
gel filtration, recombinant enzyme
Escherichia coli
86000
-
gel filtration, recombinant enzyme
Shewanella sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + acetate
Escherichia coli
-
ADP + acetyl phosphate
-
-
r
ATP + acetate
Shewanella sp.
-
ADP + acetyl phosphate
-
-
r
ATP + acetate
Shewanella sp. AS-11
-
ADP + acetyl phosphate
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by affinity chromatography and anion exchange chromatography
Escherichia coli
recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by affinity chromatography and anion exchange chromatography
Shewanella sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl phosphate + hydroxylamine
-
739591
Escherichia coli
acetyl hydroxamate + phosphate
-
-
-
?
acetyl phosphate + hydroxylamine
-
739591
Shewanella sp.
acetyl hydroxamate + phosphate
-
-
-
?
acetyl phosphate + hydroxylamine
-
739591
Shewanella sp. AS-11
acetyl hydroxamate + phosphate
-
-
-
?
ATP + acetate
-
739591
Escherichia coli
ADP + acetyl phosphate
-
-
-
r
ATP + acetate
-
739591
Shewanella sp.
ADP + acetyl phosphate
-
-
-
r
ATP + acetate
the equilibrium lies far to the left side
739591
Escherichia coli
ADP + acetyl phosphate
-
-
-
r
ATP + acetate
-
739591
Shewanella sp. AS-11
ADP + acetyl phosphate
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 42000, recombinant enzyme, SDS-PAGE, 2 * 43300, about, sequence calculation
Escherichia coli
homodimer
2 * 43000, recombinant enzyme, SDS-PAGE, 2 * 43700, about, sequence calculation
Shewanella sp.
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
about
Shewanella sp.
70
-
about
Escherichia coli
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
20
80
purified recombinant His-tagged enzyme, pH 7.5, 50% of maximal activity remaining after 15 min at 55C for the free enzyme, at 65C for the acetate-bound enzyme, and at 70C for the ATP-bound enzyme. Free enzyme is completely stable up to 40C, inactivation of all enzymes at 80C
Escherichia coli
20
80
purified recombinant His-tagged enzyme, pH 7.5, the enzyme is completely stable up to 30C, half-life is about 40-50C, inactivation at 80C
Shewanella sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
195
-
acetate
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
205
-
ATP
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
235
-
ATP
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
245
-
acetate
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
300
-
acetate
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
305
-
ATP
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
350
-
ATP
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
385
-
acetate
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
560
-
acetate
pH 7.5, 10C, recombinant enzyme
Escherichia coli
565
-
ATP
pH 7.5, 10C, recombinant enzyme
Escherichia coli
745
-
ATP
pH 7.5, 15C, recombinant enzyme
Escherichia coli
760
-
acetate
pH 7.5, 15C, recombinant enzyme
Escherichia coli
875
-
acetyl phosphate
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
884
-
ADP
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
890
-
acetyl phosphate
pH 7.5, 10C, recombinant enzyme
Escherichia coli
892
-
ADP
pH 7.5, 10C, recombinant enzyme
Escherichia coli
1130
-
ATP
pH 7.5, 20C, recombinant enzyme
Escherichia coli
1165
-
acetate
pH 7.5, 20C, recombinant enzyme
Escherichia coli
1340
-
acetyl phosphate
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
1350
-
ADP
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
1420
-
acetate
pH 7.5, 25C, recombinant enzyme
Escherichia coli
1500
-
ATP
pH 7.5, 25C, recombinant enzyme
Escherichia coli
1550
-
acetyl phosphate
pH 7.5, 15C, recombinant enzyme
Escherichia coli
1565
-
ADP
pH 7.5, 15C, recombinant enzyme
Escherichia coli
1700
-
acetyl phosphate
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
1700
-
ADP
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
2100
-
acetyl phosphate
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
2135
-
ADP
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
2150
-
acetyl phosphate
pH 7.5, 20C, recombinant enzyme
Escherichia coli
2178
-
ADP
pH 7.5, 20C, recombinant enzyme
Escherichia coli
3515
-
ADP
pH 7.5, 25C, recombinant enzyme
Escherichia coli
3600
-
acetyl phosphate
pH 7.5, 25C, recombinant enzyme
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Escherichia coli
7.5
-
assay at
Shewanella sp.
General Information
General Information
Commentary
Organism
evolution
the enzyme is found in anaerobic as well as aerobic bacteria and domain of Archaea, and play a central role in major link in the carbon cycle. The enzyme is also participated in the production of major amount o ATP in most of the fermentative anaerobes. The enzyme belongs to the phosphotransferases of the ASKHA (acetate and sugar kinases/Hsc70/actin) and the best candidate for the common ancestor of this family
Escherichia coli
evolution
the enzyme is found in anaerobic as well as aerobic bacteria and Archaea. The enzyme belongs to the ASKHA phosphotransferase family (acetate and sugar kinases/Hsc70/actin) and is the best candidate for the common ancestor of this family
Shewanella sp.
metabolism
the enzyme plays a central role in major link in the carbon cycle. The enzyme also participates in the production of major amount of ATP in most of the fermentative anaerobes
Shewanella sp.
additional information
salts bridges and cation-pi interactions in the enzyme, overview
Escherichia coli
additional information
salts bridges and cation-pi interactions in the enzyme, overview
Shewanella sp.
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme is found in anaerobic as well as aerobic bacteria and domain of Archaea, and play a central role in major link in the carbon cycle. The enzyme is also participated in the production of major amount o ATP in most of the fermentative anaerobes. The enzyme belongs to the phosphotransferases of the ASKHA (acetate and sugar kinases/Hsc70/actin) and the best candidate for the common ancestor of this family
Escherichia coli
evolution
the enzyme is found in anaerobic as well as aerobic bacteria and Archaea. The enzyme belongs to the ASKHA phosphotransferase family (acetate and sugar kinases/Hsc70/actin) and is the best candidate for the common ancestor of this family
Shewanella sp.
metabolism
the enzyme plays a central role in major link in the carbon cycle. The enzyme also participates in the production of major amount of ATP in most of the fermentative anaerobes
Shewanella sp.
additional information
salts bridges and cation-pi interactions in the enzyme, overview
Escherichia coli
additional information
salts bridges and cation-pi interactions in the enzyme, overview
Shewanella sp.
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
15.5
-
acetate
pH 7.5, 10C, recombinant enzyme
Escherichia coli
23.5
-
acetate
pH 7.5, 15C, recombinant enzyme
Escherichia coli
37.2
-
acetate
pH 7.5, 20C, recombinant enzyme
Escherichia coli
48.5
-
acetate
pH 7.5, 25C, recombinant enzyme
Escherichia coli
127
-
acetate
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
144
-
acetate
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
178
-
acetate
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
198
-
acetate
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
390
-
ATP
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
510
-
ATP
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
625
-
ATP
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
785
-
ATP
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
869
-
ATP
pH 7.5, 10C, recombinant enzyme
Escherichia coli
1164
-
ATP
pH 7.5, 15C, recombinant enzyme
Escherichia coli
1290
-
acetyl phosphate
pH 7.5, 10C, recombinant enzyme
Escherichia coli
1351
-
ADP
pH 7.5, 10C, recombinant enzyme
Escherichia coli
1650
-
acetyl phosphate
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
1768
-
ADP
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
1982
-
ATP
pH 7.5, 20C, recombinant enzyme
Escherichia coli
2183
-
acetyl phosphate
pH 7.5, 15C, recombinant enzyme
Escherichia coli
2445
-
ADP
pH 7.5, 15C, recombinant enzyme
Escherichia coli
2528
-
acetyl phosphate
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
2542
-
ATP
pH 7.5, 25C, recombinant enzyme
Escherichia coli
2700
-
ADP
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
3207
-
acetyl phosphate
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
3209
-
acetyl phosphate
pH 7.5, 20C, recombinant enzyme
Escherichia coli
3470
-
ADP
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
3513
-
ADP
pH 7.5, 20C, recombinant enzyme
Escherichia coli
4200
-
acetyl phosphate
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
4542
-
ADP
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
5762
-
ADP
pH 7.5, 25C, recombinant enzyme
Escherichia coli
6102
-
acetyl phosphate
pH 7.5, 25C, recombinant enzyme
Escherichia coli
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
15.5
-
acetate
pH 7.5, 10C, recombinant enzyme
Escherichia coli
23.5
-
acetate
pH 7.5, 15C, recombinant enzyme
Escherichia coli
37.2
-
acetate
pH 7.5, 20C, recombinant enzyme
Escherichia coli
48.5
-
acetate
pH 7.5, 25C, recombinant enzyme
Escherichia coli
127
-
acetate
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
144
-
acetate
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
178
-
acetate
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
198
-
acetate
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
390
-
ATP
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
510
-
ATP
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
625
-
ATP
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
785
-
ATP
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
869
-
ATP
pH 7.5, 10C, recombinant enzyme
Escherichia coli
1164
-
ATP
pH 7.5, 15C, recombinant enzyme
Escherichia coli
1290
-
acetyl phosphate
pH 7.5, 10C, recombinant enzyme
Escherichia coli
1351
-
ADP
pH 7.5, 10C, recombinant enzyme
Escherichia coli
1650
-
acetyl phosphate
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
1768
-
ADP
pH 7.5, 10C, recombinant enzyme
Shewanella sp.
1982
-
ATP
pH 7.5, 20C, recombinant enzyme
Escherichia coli
2183
-
acetyl phosphate
pH 7.5, 15C, recombinant enzyme
Escherichia coli
2445
-
ADP
pH 7.5, 15C, recombinant enzyme
Escherichia coli
2528
-
acetyl phosphate
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
2542
-
ATP
pH 7.5, 25C, recombinant enzyme
Escherichia coli
2700
-
ADP
pH 7.5, 15C, recombinant enzyme
Shewanella sp.
3207
-
acetyl phosphate
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
3209
-
acetyl phosphate
pH 7.5, 20C, recombinant enzyme
Escherichia coli
3470
-
ADP
pH 7.5, 20C, recombinant enzyme
Shewanella sp.
3513
-
ADP
pH 7.5, 20C, recombinant enzyme
Escherichia coli
4200
-
acetyl phosphate
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
4542
-
ADP
pH 7.5, 25C, recombinant enzyme
Shewanella sp.
5762
-
ADP
pH 7.5, 25C, recombinant enzyme
Escherichia coli
6102
-
acetyl phosphate
pH 7.5, 25C, recombinant enzyme
Escherichia coli
Other publictions for EC 2.7.2.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730304
Ingram-Smith
The role of active site residu ...
Methanosarcina thermophila
Life
5
861-871
2015
-
-
1
-
7
-
-
29
-
-
-
-
-
2
-
-
1
-
-
-
-
-
6
-
2
-
-
23
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
29
-
-
-
-
-
-
-
1
-
-
-
-
6
-
2
-
-
23
-
-
-
-
-
-
-
-
22
22
737465
Rozova
Acetate kinase-an enzyme of th ...
Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum
Antonie van Leeuwenhoek
108
965-974
2015
-
-
1
-
-
-
-
5
-
4
2
2
-
8
-
-
1
-
-
-
-
-
4
1
1
1
1
4
1
1
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
5
-
4
2
2
-
-
-
1
-
-
-
-
4
1
1
1
1
4
1
1
-
-
-
1
1
-
-
-
738863
Zhang
Enzymatic manufacture of deoxy ...
Escherichia coli
J. Microbiol. Biotechnol.
25
2034-2042
2015
-
-
1
-
1
-
-
-
-
1
-
1
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
746769
Rozova
Acetate kinase-an enzyme of t ...
Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum
Antonie van Leeuwenhoek
108
965-974
2015
-
-
1
-
-
-
-
2
-
3
3
-
-
8
-
-
1
-
-
-
-
-
4
1
1
2
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
3
3
-
-
-
-
1
-
-
-
-
4
1
1
2
1
2
1
-
-
-
-
-
-
-
2
2
727558
Welte
Experimental evidence of an ac ...
Methanosarcina mazei, Methanosarcina mazei DSM 3647
FEMS Microbiol. Lett.
359
147-153
2014
-
-
1
-
-
-
-
1
-
-
-
2
-
4
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
738528
Puri
Regulation of acetate kinase i ...
Lactococcus lactis, Lactococcus lactis MG1363
J. Bacteriol.
196
1386-1393
2014
-
-
1
-
-
-
4
8
-
3
-
4
-
5
-
-
1
-
-
-
-
-
4
-
1
-
-
4
1
-
-
2
7
-
-
-
-
2
4
-
-
-
-
8
11
10
-
6
-
4
-
-
-
2
-
-
-
-
4
-
2
-
-
4
2
-
-
-
-
1
2
-
-
-
738717
Yoshioka
Structural and mutational anal ...
Escherichia coli
J. Biosci. Bioeng.
118
502-507
2014
-
-
1
-
7
-
-
8
-
1
-
1
-
3
-
-
1
-
-
-
-
-
3
-
1
-
-
7
1
-
-
3
-
-
-
-
-
1
3
-
7
-
-
-
-
8
-
1
-
1
-
-
-
1
-
-
-
-
3
-
1
-
-
7
1
-
-
-
-
2
2
-
-
-
739218
Kushkevych
Acetate kinase activity and ki ...
Desulfomicrobium sp., Desulfomicrobium sp. Rod-9, Desulfovibrio piger, Desulfovibrio piger Vib-7
Open Microbiol. J.
8
138-143
2014
-
-
-
-
-
-
-
2
2
2
-
4
-
16
-
-
-
-
-
2
2
-
4
-
2
2
-
-
2
2
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
2
2
2
-
4
-
-
-
-
-
2
2
-
4
-
2
2
-
-
2
2
-
-
-
2
2
-
-
-
739591
Tang
Cold adaptation: structural an ...
Escherichia coli, Shewanella sp., Shewanella sp. AS-11
Protein J.
33
313-322
2014
-
-
2
-
-
-
-
30
-
3
6
3
-
6
-
-
2
-
-
-
-
-
7
2
2
-
2
32
2
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
30
-
3
6
3
-
-
-
2
-
-
-
-
7
2
2
-
2
32
2
-
-
-
-
5
5
-
32
32
723068
Thaker
Crystal structures of acetate ...
Cryptococcus neoformans, Entamoeba histolytica
J. Struct. Biol.
181
185-189
2013
-
-
2
2
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738854
Asgari
Targeting acetate kinase: inhi ...
Escherichia coli
J. Microbiol. Biotechnol.
23
1544-1553
2013
-
-
-
-
-
-
3
-
-
1
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721949
Chittori
Structural and mechanistic inv ...
Salmonella enterica subsp. enterica serovar Typhimurium
BMC Struct. Biol.
12
24
2012
-
-
1
1
-
-
5
11
-
4
1
-
-
4
-
-
1
-
-
-
-
1
6
-
1
1
-
8
1
1
-
-
-
-
5
-
-
1
-
1
-
-
5
5
-
11
-
4
1
-
-
-
-
1
-
-
-
1
6
-
1
1
-
8
1
1
-
-
-
-
-
-
8
8
721205
Chittori
Preliminary X-ray crystallogra ...
Salmonella enterica subsp. enterica serovar Typhimurium
Acta Crystallogr. Sect. F
67
1658-1661
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722528
Ramos-Montanez
Instability of ackA (acetate k ...
Streptococcus pneumoniae
J. Bacteriol.
192
6390-6400
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
691204
Mukhopadhyay
A continuous assay of acetate ...
Thermotoga maritima
Bioorg. Chem.
36
65-69
2008
-
-
-
-
-
-
1
1
-
-
-
1
-
2
-
-
1
-
-
-
1
-
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
1
-
-
1
-
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
673209
Ren
Cloning, expression, and chara ...
Ethanoligenens harbinense
Curr. Microbiol.
55
167-172
2007
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
673750
Baldock
Roseovarius sp. strain 217: ae ...
Roseovarius sp., Roseovarius sp. 217
FEMS Microbiol. Lett.
271
202-206
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690888
Gorrell
Investigation of the Methanosa ...
Methanosarcina thermophila
Biochemistry
46
14170-14176
2007
-
-
1
-
5
-
-
22
-
1
-
1
-
2
-
-
1
1
-
-
1
-
2
-
-
-
-
12
-
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
22
-
1
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
693619
Halbedel
Transcription in Mycoplasma pn ...
Mycoplasma pneumoniae
J. Mol. Biol.
371
596-607
2007
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672708
Suwannakham
Construction and characterizat ...
Acidipropionibacterium acidipropionici
Biotechnol. Bioeng.
94
383-395
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677237
Ingram-Smith
Acetate kinase: not just a bac ...
Aspergillus fumigatus, Aspergillus nidulans, Bacillus subtilis, Borreliella burgdorferi, Chaetomium globosum, Chlamydomonas reinhardtii, Coccidioides immitis, Coprinopsis cinerea, Cryptococcus neoformans, Cupriavidus necator, Entamoeba histolytica, Enterococcus faecalis, Fusarium graminearum, Helicobacter pylori, Histoplasma capsulatum, Lactobacillus acidophilus, Lactococcus lactis, Listeria monocytogenes, Magnaporthe grisea, Mesoplasma florum, Methanosarcina acetivorans, Methanosarcina mazei, Methanosarcina thermophila, Mycoplasma pneumoniae, Neurospora crassa, Oceanobacillus iheyensis, Parastagonospora nodorum, Phanerochaete chrysosporium, Phytophthora ramorum, Phytophthora sojae, Salmonella enterica, Sclerotinia sclerotiorum, Staphylococcus aureus, Streptococcus pneumoniae, Trichoderma reesei, Uncinocarpus reesii, Ustilago maydis
Trends Microbiol.
14
249-253
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
42
-
-
-
-
-
-
-
-
37
-
-
-
-
-
-
-
-
37
-
-
-
-
-
-
37
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659065
Ingram-Smith
Characterization of the acetat ...
Methanosarcina thermophila
J. Bacteriol.
187
2386-2394
2005
-
-
-
1
5
-
-
27
-
-
-
-
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
1
5
-
-
-
-
27
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
659446
Gorrell
Structural and kinetic analyse ...
Methanosarcina thermophila
J. Biol. Chem.
280
10731-10742
2005
-
-
-
1
5
-
1
32
-
-
-
-
-
2
-
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-
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13
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5
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1
32
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2
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13
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659856
Iyer
-
Acetate kinase from Methanosar ...
Methanosarcina thermophila
Methods Biotechnol.
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239-246
2005
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1
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1
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4
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9
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9
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2
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659056
Hasona
Pyruvate formate lyase and ace ...
Escherichia coli
J. Bacteriol.
186
7593-7600
2004
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1
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3
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642198
Ito
Highly sensitive simultaneous ...
Geobacillus stearothermophilus
Anal. Sci.
19
105-109
2003
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-
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1
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1
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727342
Tonouchi
Cloning of a gene encoding ace ...
Methanosarcina mazei, Methanosarcina mazei 2-P
Curr. Microbiol.
45
390-393
2002
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1
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2
2
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5
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1
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4
1
1
1
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1
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1
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2
2
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1
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4
1
1
1
-
-
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1
-
1
1
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-
727839
Miles
Evidence for a transition stat ...
Methanosarcina thermophila
J. Biol. Chem.
277
22547-22552
2002
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1
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11
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2
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1
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1
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1
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8
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1
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11
8
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1
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1
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1
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642195
Knorr
Cloning, expression, and chara ...
Lactobacillus sanfranciscensis
Microbiol. Res.
156
267-277
2001
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1
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4
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2
2
1
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4
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1
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1
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2
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1
1
1
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1
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1
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4
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2
2
1
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1
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1
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2
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1
1
1
-
1
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-
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-
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642196
Miles
Site-directed mutational analy ...
Methanosarcina thermophila
J. Biol. Chem.
276
45059-45064
2001
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1
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11
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28
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3
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1
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2
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9
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1
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1
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11
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28
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3
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1
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9
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1
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642197
Yu
Purification and characterizat ...
Desulfovibrio vulgaris, Desulfovibrio vulgaris Miyazaki F.
J. Biochem.
129
411-421
2001
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-
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1
1
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1
3
2
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4
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1
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1
2
1
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1
1
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1
3
2
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1
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2
1
2
1
-
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1
1
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-
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-
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727737
Buss
Urkinase: structure of acetate ...
Methanosarcina thermophila
J. Bacteriol.
183
680-686
2001
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1
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1
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1
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1
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1
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1
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-
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642193
Ingram-Smith
The role of histidines in the ...
Methanosarcina thermophila
J. Biol. Chem.
275
33765-33770
2000
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1
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9
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1
29
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2
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1
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1
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9
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1
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29
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1
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-
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642194
Singh-Wissmann
Identification of essential ar ...
Methanosarcina thermophila
Biochemistry
39
3671-3677
2000
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1
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7
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1
19
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1
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3
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1
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1
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1
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7
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1
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19
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1
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1
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1
-
-
-
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-
-
-
-
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487537
Bock
Purification and characterizat ...
Thermotoga maritima
J. Bacteriol.
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1861-1867
1999
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1
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4
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6
2
1
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7
1
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1
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1
7
1
1
1
1
-
1
1
-
-
-
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-
1
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4
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6
2
1
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1
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1
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1
7
1
1
1
1
-
1
1
-
-
-
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-
-
-
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642190
Lin
-
Purification and characterizat ...
Hungateiclostridium thermocellum
Tetrahedron
54
15915-15925
1998
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1
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1
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2
2
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1
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1
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1
1
1
1
1
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1
1
1
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1
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1
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2
2
1
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1
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1
-
1
1
1
1
1
-
1
1
1
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-
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642191
Singh-Wissmann
Identification of essential gl ...
Methanosarcina thermophila
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1129-1134
1998
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3
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2
21
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1
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1
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1
1
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1
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3
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2
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21
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1
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1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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642188
Buss
Crystallization of acetate kin ...
Methanosarcina thermophila
Protein Sci.
6
2659-2662
1997
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642189
Winzer
-
Acetate kinase from Clostridiu ...
Clostridium acetobutylicum
Microbiology
143
3279-3286
1997
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-
1
-
-
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2
4
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4
3
1
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1
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1
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1
-
7
1
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1
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2
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4
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4
3
1
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1
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1
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642187
Diez-Gonzalez
The acetate kinase of Clostrid ...
Clostridium acetobutylicum, Clostridium acetobutylicum P262
Arch. Microbiol.
166
418-420
1996
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-
-
-
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1
3
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2
2
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3
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1
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1
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4
1
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4
1
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1
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3
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2
2
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1
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1
-
4
1
-
-
-
4
1
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-
-
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642184
Aceti
-
Purification and characterizat ...
Methanosarcina thermophila
J. Biol. Chem.
249
15444-15448
1988
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-
-
-
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2
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2
1
4
2
1
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1
1
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1
-
-
-
1
1
8
1
-
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
2
1
4
2
1
-
1
-
1
-
-
1
1
8
1
-
1
1
-
1
1
-
1
-
-
-
-
-
-
642185
Kim
Inactivation of Acinetobacter ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
956
103-109
1988
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-
-
-
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1
2
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-
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1
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2
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1
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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1
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2
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1
-
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1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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642186
Mannens
-
Purification and immobilizatio ...
Desulfovibrio vulgaris
Biotechnol. Lett.
10
563-568
1988
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
-
1
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-
-
-
-
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-
-
-
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-
-
-
-
-
-
-
-
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-
-
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-
-
1
-
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-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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727804
Aceti
Purification and characterizat ...
Methanosarcina thermophila, Methanosarcina thermophila TM-1
J. Biol. Chem.
263
15444-15448
1988
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-
-
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1
1
2
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4
3
2
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3
1
-
1
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-
1
-
1
14
1
1
-
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
2
-
4
3
2
-
1
-
1
-
1
-
1
14
1
1
-
1
-
1
1
-
1
-
1
1
-
-
-
642182
Fox
Isolation and characterization ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
J. Biol. Chem.
261
13487-13497
1986
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2
6
11
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4
3
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44
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2
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-
-
-
1
10
2
-
-
-
-
2
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-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
11
-
-
4
3
-
-
-
2
-
-
-
1
10
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
642183
Vigenschow
Purification and properties of ...
Rhodopseudomonas palustris
Biol. Chem. Hoppe-Seyler
367
951-956
1986
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-
-
-
-
-
-
11
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2
4
1
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2
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1
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-
-
1
2
8
1
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
11
-
2
4
1
-
-
-
1
-
-
1
2
8
1
-
-
1
-
1
-
-
1
-
-
-
-
-
-
642181
Ichikawa
-
Purification and properties of ...
Propionibacterium freudenreichii
J. Ferment. Technol.
63
143-149
1985
-
-
-
-
-
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-
6
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6
2
1
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1
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1
-
-
-
1
-
5
1
1
-
1
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
6
2
1
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-
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1
-
-
1
-
5
1
1
-
1
-
2
1
1
-
-
-
-
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-
-
642179
Harwood
Properties of acetate kinase i ...
Spirochaeta sp., Spirochaeta sp. MA-2
J. Bacteriol.
152
246-254
1982
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-
-
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6
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6
2
2
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2
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12
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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6
-
6
2
2
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-
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-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642180
Suzuki
-
Acetate kinase from Bacillus s ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus NCA 1503
Methods Enzymol.
90
179-185
1982
3
-
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1
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3
2
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13
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1
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1
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10
1
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1
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1
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1
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-
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3
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-
-
1
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-
-
-
-
-
-
3
2
2
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1
-
-
1
-
10
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
642178
Nishimura
-
Acetate kinase from Veilonella ...
Veillonella parvula, Veillonella parvula 221 / ATCC 17745
Methods Enzymol.
71
311-316
1981
1
-
-
-
-
-
-
-
-
3
2
2
-
2
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1
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-
-
1
1
10
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
2
2
-
-
-
1
-
-
1
1
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642177
Wong
Inactivation of Escherichia co ...
Escherichia coli
Biochim. Biophys. Acta
615
121-131
1980
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-
-
-
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1
3
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1
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2
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-
-
-
-
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
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-
-
-
-
1
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
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642174
Griffith
Acetate kinase from Veillonell ...
Veillonella parvula
J. Biol. Chem.
254
442-446
1979
1
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-
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5
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2
1
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2
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1
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1
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1
1
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-
-
-
1
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-
-
-
-
-
1
-
-
-
-
-
-
-
5
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-
-
-
2
1
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-
-
1
-
-
1
-
1
1
-
-
-
-
1
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-
-
-
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-
-
-
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642175
Griffith
Acetate kinase from Veillonell ...
Veillonella parvula
J. Biol. Chem.
254
6698-6702
1979
1
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-
2
4
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
642176
Kahane
Purification and properties of ...
Acholeplasma laidlawii, Acholeplasma laidlawii oral
J. Bacteriol.
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764-772
1979
-
-
-
-
-
1
6
6
-
1
5
2
-
3
-
-
1
-
-
-
1
1
11
1
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
6
-
1
5
2
-
-
-
1
-
-
1
1
11
1
-
-
-
5
-
-
-
-
-
-
-
-
-
-
642172
Nakajima
Purification and properties of ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus NCA 1503
J. Biochem.
84
193-203
1978
-
-
-
1
-
1
2
-
-
6
3
2
-
14
1
-
1
-
-
-
1
-
11
1
-
-
1
-
1
1
1
-
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
6
3
2
-
1
-
1
-
-
1
-
11
1
-
-
1
-
1
1
1
-
-
-
-
-
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-
642173
Yoshimura
Purification and characterizat ...
Veillonella parvula
Arch. Biochem. Biophys.
189
424-432
1978
1
-
-
-
-
-
9
4
-
5
2
1
-
3
-
-
1
-
-
-
1
2
5
1
-
-
1
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
9
-
4
-
5
2
1
-
-
-
1
-
-
1
2
5
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
642171
Bowman
Acetate kinase from Veillonell ...
Veillonella parvula
J. Biol. Chem.
251
3117-3121
1976
1
-
-
-
-
-
-
10
-
-
2
1
-
2
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
10
-
-
2
1
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
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-
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-
642168
Janson
The inhibition of acetate, pyr ...
Escherichia coli
J. Biol. Chem.
249
2567-2571
1974
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
-
-
-
-
-
-
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-
-
-
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-
-
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-
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-
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-
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2
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1
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-
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-
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-
642169
Janson
The specificity of chromium nu ...
Escherichia coli
J. Biol. Chem.
249
2572-2574
1974
-
-
-
-
-
-
1
-
-
1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
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-
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1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642170
Schaupp
Purification and properties of ...
Moorella thermoacetica
Arch. Microbiol.
100
121-129
1974
-
-
-
-
-
-
-
1
-
-
1
1
-
2
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
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-
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-
-
-
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-
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1
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-
1
1
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1
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-
-
-
1
-
1
-
-
-
-
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-
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642167
Anthony
Exchange reactions catalyzed b ...
Escherichia coli
J. Biol. Chem.
246
6129-6135
1971
-
-
-
-
-
-
-
-
-
1
-
1
-
1
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-
1
-
-
-
-
1
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
1
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
642166
Rose
-
Acetate kinase ...
Escherichia coli, Lactobacillus delbrueckii, Streptococcus pyogenes
The Enzymes, 2nd. Ed. (Boyer, P. D. , Lardy, H. , Myrbaeck, K. , eds. )
6
115-118
1962
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1
3
4
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1
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3
-
3
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2
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1
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7
-
-
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1
-
1
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1
-
3
-
4
-
1
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3
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2
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1
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7
-
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1
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1
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