Literature summary for 2.7.2.1 extracted from

Zhang, J.; Qian, Y.; Ding, Q.; Ou, L.
Enzymatic manufacture of deoxythymidine-5-triphosphate with permeable intact cells of E. coli coexpressing thymidylate kinase and acetate kinase (2015), J. Microbiol. Biotechnol., 25, 2034-2042.

Search for more literature for 2.7.2.1

Cloned(Commentary)

Cloned (Comment)	Organism
gene ackA, recombinant expression in Escherichia coli strain BL21(DE3), co-overexpression with Escherichia coli thymidylate kinase, EC 2.7.4.9, resulting in a large number of inclusion bodies	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
additional information	development of a one-pot process of enzymatic synthesis of deoxythymidine-5'-triphosphate (5'-dTTP) employing whole cells of recombinant Escherichia coli coexpressing thymidylate kinase (TMKase, EC 2.7.4.9) and acetate kinase (ACKase). The relative residual specific activities of TMKase and ACKase, pretreated with 20 mM EDTA, are 94% and 96%, respectively. The yield of 5'-dTTP reaches above 94% from 5 mM 5'-dTMP and 15 mM acetyl phosphate catalyzed with intact cells of the recombinant strain pretreated with EDTA. The process is so effective that only 0.125 mM ATP is sufficient to deliver the phosphate group from acetyl phosphate to dTMP and dTDP	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + acetate	Escherichia coli	-	ADP + acetyl phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A6A3	gene ackA	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + acetate	-	Escherichia coli	ADP + acetyl phosphate	-	r

Synonyms

Synonyms	Comment	Organism
ackA	-	Escherichia coli
ACKase	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ADP	-	Escherichia coli
ATP	-	Escherichia coli

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Release 2023.1 (January 2023)