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Literature summary for 2.7.2.1 extracted from

  • Miles, R.D.; Gorrell, A.; Ferry, J.G.
    Evidence for a transition state analog, MgADP-aluminum fluoride-acetate, in acetate kinase from Methanosarcina thermophila (2002), J. Biol. Chem., 277, 22547-22552.
    View publication on PubMed
Search for more literature for 2.7.2.1

Cloned(Commentary)

Cloned (Comment) Organism
recombinant acetate kinase with a 6-residue N-terminal histidine tag is heterologously produced in Escherichia coli BL21(DE3) Methanosarcina thermophila

Inhibitors

Inhibitors Comment Organism Structure
acetate inhibition by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate. When MgCl2, ADP, and acetate are omitted from the preincubation mixture, there is no detectable loss of activity; inhibition of acetate kinase by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate (all of the components are necessary for maximum inhibition) Methanosarcina thermophila
ADP inhibition by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate. When MgCl2, ADP, and acetate are omitted from the preincubation mixture, there is no detectable loss of activity; inhibition of acetate kinase by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate (all of the components are necessary for maximum inhibition). The transition state analog, MgADP-aluminum fluoride-acetate, forms an abortive complex in the active site Methanosarcina thermophila
AlCl3 inhibition by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate. When MgCl2, ADP, and acetate are omitted from the preincubation mixture, there is no detectable loss of activity; inhibition of acetate kinase by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate (all of the components are necessary for maximum inhibition). The transition state analog, MgADP-aluminum fluoride-acetate, forms an abortive complex in the active site. Protection from inhibition by a non-hydrolyzable ATP analog or acetylphosphate. Preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity Methanosarcina thermophila
ATP-gamma-S non-hydrolyzable inhibitor Methanosarcina thermophila
CDP preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity Methanosarcina thermophila
IDP preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity Methanosarcina thermophila
MgCl2 inhibition by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate. When MgCl2, ADP, and acetate are omitted from the preincubation mixture, there is no detectable loss of activity; inhibition of acetate kinase by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate (all of the components are necessary for maximum inhibition). The transition state analog, MgADP-aluminum fluoride-acetate, forms an abortive complex in the active site. Preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity Methanosarcina thermophila
additional information preincubation with butyrate does not significantly inhibit the enzyme Methanosarcina thermophila
NaF inhibition by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate. When MgCl2, ADP, and acetate are omitted from the preincubation mixture, there is no detectable loss of activity; inhibition of acetate kinase by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate (all of the components are necessary for maximum inhibition). The transition state analog, MgADP-aluminum fluoride-acetate, forms an abortive complex in the active site. Preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity Methanosarcina thermophila
propionate preincubation with MgCl2, ADP, AlCl3, NaF, and propionate results in almost complete inhibition of activity Methanosarcina thermophila
UDP preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity Methanosarcina thermophila

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila P38502
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate
-
 Methanosarcina thermophila ADP + acetyl phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Methanosarcina thermophila

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0184
-
 ADP pH and temperature not specified in the publication Methanosarcina thermophila
0.95
-
 AlCl3 pH and temperature not specified in the publication Methanosarcina thermophila
0.95
-
 MgCl2 pH 5.5, temperature not specified in the publication Methanosarcina thermophila
2.9
-
 acetate pH and temperature not specified in the publication Methanosarcina thermophila
2.9
-
 acetate pH 5.5, temperature not specified in the publication Methanosarcina thermophila
3
-
 NaF pH and temperature not specified in the publication Methanosarcina thermophila
3.4
-
 MgCl2 pH and temperature not specified in the publication Methanosarcina thermophila
3.4
-
 AlCl3 pH 5.5, temperature not specified in the publication Methanosarcina thermophila
18.4
-
 ADP pH 5.5, temperature not specified in the publication Methanosarcina thermophila