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Literature summary for 2.7.2.1 extracted from

  • Welte, C.; Kröninger, L.; Deppenmeier, U.
    Experimental evidence of an acetate transporter protein and characterization of acetate activation in aceticlastic methanogenesis of Methanosarcina mazei (2014), FEMS Microbiol. Lett., 359, 147-153.
    View publication on PubMed
Search for more literature for 2.7.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanosarcina mazei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1 2 acetate pH 7.0, temperature not specified in the publication Methanosarcina mazei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate Methanosarcina mazei
-
 ADP + acetyl phosphate
-
 ?
ATP + acetate Methanosarcina mazei DSM 3647
-
 ADP + acetyl phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina mazei P0CW05
-
-
Methanosarcina mazei DSM 3647 P0CW05
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7
-
 cell lysate, pH 7.0, temperature not specified in the publication Methanosarcina mazei
251
-
 isolated enzyme, pH 7.0, temperature not specified in the publication Methanosarcina mazei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate
-
 Methanosarcina mazei ADP + acetyl phosphate
-
 ?
ATP + acetate
-
 Methanosarcina mazei DSM 3647 ADP + acetyl phosphate
-
 ?

Synonyms

Synonyms Comment Organism
MM_0495 locus name Methanosarcina mazei

General Information

General Information Comment Organism
physiological function at a concentration of 2 mM acetate, the acetate kinase activity in cell extract of Methanosarcina mazei is not limiting the methane formation rate Methanosarcina mazei