BRENDA - Enzyme Database show
show all sequences of 2.7.2.1

A continuous assay of acetate kinase activity: measurement of inorganic phosphate release generated by hydroxylaminolysis of acetyl phosphate

Mukhopadhyay, S.; Hasson, M.S.; Sanders, D.A.; Bioorg. Chem. 36, 65-69 (2008)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
hydroxylamine
inhibits acetate kinase reaction in a nonlinear and noncompetitive fashion, substantial inhibition at concentrations of 704 mM and minimal inhibition at concentrations of 250 microM hydroxylamine
Thermotoga maritima
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
lower Km of the Thermotoga maritima acetate kinase for acetate measured than that determined by earlier assay methods
Thermotoga maritima
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + acetate
Thermotoga maritima
-
ADP + acetyl phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermotoga maritima
Q9WYB1
-
-
Purification (Commentary)
Commentary
Organism
gel filtration
Thermotoga maritima
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
continuous assay, spectrophotometric quantification of phosphate achieved through measurement of the phosphorylysis of 2-amino-6-mercapto-7-methyl-purine riboside (MESG) to ribose-1-phosphate and 2-amino-6-mercapto-7-methyl-purine (MES) by purine nucleoside phosphorylase, sensitivity of the assay is in the range of 2-150 microM
Thermotoga maritima
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl phosphate + hydroxylamine
high-energy acetyl phosphate reacts with hydroxylamine forming acetyl hydroxamate and inorganic phosphate
691204
Thermotoga maritima
acetyl hydroxamate + phosphate
-
-
-
?
ATP + acetate
-
691204
Thermotoga maritima
ADP + acetyl phosphate
-
-
-
?
ATP + acetate
enzyme assay developed that can measure the released phosphate at a nanomolar level
691204
Thermotoga maritima
ADP + acetyl phosphate
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Thermotoga maritima
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Thermotoga maritima
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
assay can be performed over pH range of
Thermotoga maritima
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
hydroxylamine
inhibits acetate kinase reaction in a nonlinear and noncompetitive fashion, substantial inhibition at concentrations of 704 mM and minimal inhibition at concentrations of 250 microM hydroxylamine
Thermotoga maritima
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
lower Km of the Thermotoga maritima acetate kinase for acetate measured than that determined by earlier assay methods
Thermotoga maritima
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + acetate
Thermotoga maritima
-
ADP + acetyl phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
gel filtration
Thermotoga maritima
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
continuous assay, spectrophotometric quantification of phosphate achieved through measurement of the phosphorylysis of 2-amino-6-mercapto-7-methyl-purine riboside (MESG) to ribose-1-phosphate and 2-amino-6-mercapto-7-methyl-purine (MES) by purine nucleoside phosphorylase, sensitivity of the assay is in the range of 2-150 microM
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl phosphate + hydroxylamine
high-energy acetyl phosphate reacts with hydroxylamine forming acetyl hydroxamate and inorganic phosphate
691204
Thermotoga maritima
acetyl hydroxamate + phosphate
-
-
-
?
ATP + acetate
-
691204
Thermotoga maritima
ADP + acetyl phosphate
-
-
-
?
ATP + acetate
enzyme assay developed that can measure the released phosphate at a nanomolar level
691204
Thermotoga maritima
ADP + acetyl phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Thermotoga maritima
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Thermotoga maritima
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
assay can be performed over pH range of
Thermotoga maritima
Other publictions for EC 2.7.2.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730304
Ingram-Smith
The role of active site residu ...
Methanosarcina thermophila
Life
5
861-871
2015
-
-
1
-
7
-
-
29
-
-
-
-
-
2
-
-
1
-
-
-
-
-
6
-
2
-
-
23
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
29
-
-
-
-
-
-
-
1
-
-
-
-
6
-
2
-
-
23
-
-
-
-
-
-
-
-
22
22
737465
Rozova
Acetate kinase-an enzyme of th ...
Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum
Antonie van Leeuwenhoek
108
965-974
2015
-
-
1
-
-
-
-
5
-
4
2
2
-
8
-
-
1
-
-
-
-
-
4
1
1
1
1
4
1
1
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
5
-
4
2
2
-
-
-
1
-
-
-
-
4
1
1
1
1
4
1
1
-
-
-
1
1
-
-
-
738863
Zhang
Enzymatic manufacture of deoxy ...
Escherichia coli
J. Microbiol. Biotechnol.
25
2034-2042
2015
-
-
1
-
1
-
-
-
-
1
-
1
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
746769
Rozova
Acetate kinase-an enzyme of t ...
Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum
Antonie van Leeuwenhoek
108
965-974
2015
-
-
1
-
-
-
-
2
-
3
3
-
-
8
-
-
1
-
-
-
-
-
4
1
1
2
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
3
3
-
-
-
-
1
-
-
-
-
4
1
1
2
1
2
1
-
-
-
-
-
-
-
2
2
727558
Welte
Experimental evidence of an ac ...
Methanosarcina mazei, Methanosarcina mazei DSM 3647
FEMS Microbiol. Lett.
359
147-153
2014
-
-
1
-
-
-
-
1
-
-
-
2
-
4
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
738528
Puri
Regulation of acetate kinase i ...
Lactococcus lactis, Lactococcus lactis MG1363
J. Bacteriol.
196
1386-1393
2014
-
-
1
-
-
-
4
8
-
3
-
4
-
5
-
-
1
-
-
-
-
-
4
-
1
-
-
4
1
-
-
2
7
-
-
-
-
2
4
-
-
-
-
8
11
10
-
6
-
4
-
-
-
2
-
-
-
-
4
-
2
-
-
4
2
-
-
-
-
1
2
-
-
-
738717
Yoshioka
Structural and mutational anal ...
Escherichia coli
J. Biosci. Bioeng.
118
502-507
2014
-
-
1
-
7
-
-
8
-
1
-
1
-
3
-
-
1
-
-
-
-
-
3
-
1
-
-
7
1
-
-
3
-
-
-
-
-
1
3
-
7
-
-
-
-
8
-
1
-
1
-
-
-
1
-
-
-
-
3
-
1
-
-
7
1
-
-
-
-
2
2
-
-
-
739218
Kushkevych
Acetate kinase activity and ki ...
Desulfomicrobium sp., Desulfomicrobium sp. Rod-9, Desulfovibrio piger, Desulfovibrio piger Vib-7
Open Microbiol. J.
8
138-143
2014
-
-
-
-
-
-
-
2
2
2
-
4
-
16
-
-
-
-
-
2
2
-
4
-
2
2
-
-
2
2
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
2
2
2
-
4
-
-
-
-
-
2
2
-
4
-
2
2
-
-
2
2
-
-
-
2
2
-
-
-
739591
Tang
Cold adaptation: structural an ...
Escherichia coli, Shewanella sp., Shewanella sp. AS-11
Protein J.
33
313-322
2014
-
-
2
-
-
-
-
30
-
3
6
3
-
6
-
-
2
-
-
-
-
-
7
2
2
-
2
32
2
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
30
-
3
6
3
-
-
-
2
-
-
-
-
7
2
2
-
2
32
2
-
-
-
-
5
5
-
32
32
723068
Thaker
Crystal structures of acetate ...
Cryptococcus neoformans, Entamoeba histolytica
J. Struct. Biol.
181
185-189
2013
-
-
2
2
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738854
Asgari
Targeting acetate kinase: inhi ...
Escherichia coli
J. Microbiol. Biotechnol.
23
1544-1553
2013
-
-
-
-
-
-
3
-
-
1
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721949
Chittori
Structural and mechanistic inv ...
Salmonella enterica subsp. enterica serovar Typhimurium
BMC Struct. Biol.
12
24
2012
-
-
1
1
-
-
5
11
-
4
1
-
-
4
-
-
1
-
-
-
-
1
6
-
1
1
-
8
1
1
-
-
-
-
5
-
-
1
-
1
-
-
5
5
-
11
-
4
1
-
-
-
-
1
-
-
-
1
6
-
1
1
-
8
1
1
-
-
-
-
-
-
8
8
721205
Chittori
Preliminary X-ray crystallogra ...
Salmonella enterica subsp. enterica serovar Typhimurium
Acta Crystallogr. Sect. F
67
1658-1661
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722528
Ramos-Montanez
Instability of ackA (acetate k ...
Streptococcus pneumoniae
J. Bacteriol.
192
6390-6400
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
691204
Mukhopadhyay
A continuous assay of acetate ...
Thermotoga maritima
Bioorg. Chem.
36
65-69
2008
-
-
-
-
-
-
1
1
-
-
-
1
-
2
-
-
1
-
-
-
1
-
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
1
-
-
1
-
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
673209
Ren
Cloning, expression, and chara ...
Ethanoligenens harbinense
Curr. Microbiol.
55
167-172
2007
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
673750
Baldock
Roseovarius sp. strain 217: ae ...
Roseovarius sp., Roseovarius sp. 217
FEMS Microbiol. Lett.
271
202-206
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690888
Gorrell
Investigation of the Methanosa ...
Methanosarcina thermophila
Biochemistry
46
14170-14176
2007
-
-
1
-
5
-
-
22
-
1
-
1
-
2
-
-
1
1
-
-
1
-
2
-
-
-
-
12
-
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
22
-
1
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
693619
Halbedel
Transcription in Mycoplasma pn ...
Mycoplasma pneumoniae
J. Mol. Biol.
371
596-607
2007
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672708
Suwannakham
Construction and characterizat ...
Acidipropionibacterium acidipropionici
Biotechnol. Bioeng.
94
383-395
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677237
Ingram-Smith
Acetate kinase: not just a bac ...
Aspergillus fumigatus, Aspergillus nidulans, Bacillus subtilis, Borreliella burgdorferi, Chaetomium globosum, Chlamydomonas reinhardtii, Coccidioides immitis, Coprinopsis cinerea, Cryptococcus neoformans, Cupriavidus necator, Entamoeba histolytica, Enterococcus faecalis, Fusarium graminearum, Helicobacter pylori, Histoplasma capsulatum, Lactobacillus acidophilus, Lactococcus lactis, Listeria monocytogenes, Magnaporthe grisea, Mesoplasma florum, Methanosarcina acetivorans, Methanosarcina mazei, Methanosarcina thermophila, Mycoplasma pneumoniae, Neurospora crassa, Oceanobacillus iheyensis, Parastagonospora nodorum, Phanerochaete chrysosporium, Phytophthora ramorum, Phytophthora sojae, Salmonella enterica, Sclerotinia sclerotiorum, Staphylococcus aureus, Streptococcus pneumoniae, Trichoderma reesei, Uncinocarpus reesii, Ustilago maydis
Trends Microbiol.
14
249-253
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
42
-
-
-
-
-
-
-
-
37
-
-
-
-
-
-
-
-
37
-
-
-
-
-
-
37
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
37
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659065
Ingram-Smith
Characterization of the acetat ...
Methanosarcina thermophila
J. Bacteriol.
187
2386-2394
2005
-
-
-
1
5
-
-
27
-
-
-
-
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
1
5
-
-
-
-
27
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
659446
Gorrell
Structural and kinetic analyse ...
Methanosarcina thermophila
J. Biol. Chem.
280
10731-10742
2005
-
-
-
1
5
-
1
32
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
13
-
-
-
-
1
-
-
-
-
-
-
1
5
-
-
1
1
32
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
659856
Iyer
-
Acetate kinase from Methanosar ...
Methanosarcina thermophila
Methods Biotechnol.
17
239-246
2005
-
1
-
-
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
9
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
9
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
659056
Hasona
Pyruvate formate lyase and ace ...
Escherichia coli
J. Bacteriol.
186
7593-7600
2004
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642198
Ito
Highly sensitive simultaneous ...
Geobacillus stearothermophilus
Anal. Sci.
19
105-109
2003
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727342
Tonouchi
Cloning of a gene encoding ace ...
Methanosarcina mazei, Methanosarcina mazei 2-P
Curr. Microbiol.
45
390-393
2002
-
-
1
-
-
-
-
-
-
-
2
2
-
5
-
-
1
-
-
-
-
-
4
1
1
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
1
-
-
-
-
4
1
1
1
-
-
-
-
-
1
-
1
1
-
-
-
727839
Miles
Evidence for a transition stat ...
Methanosarcina thermophila
J. Biol. Chem.
277
22547-22552
2002
-
-
1
-
-
-
11
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
8
-
-
-
-
1
-
-
-
-
-
11
8
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
642195
Knorr
Cloning, expression, and chara ...
Lactobacillus sanfranciscensis
Microbiol. Res.
156
267-277
2001
-
-
1
-
-
-
-
4
-
2
2
1
-
4
-
-
1
-
-
-
1
-
2
-
1
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
2
2
1
-
-
-
1
-
-
1
-
2
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
642196
Miles
Site-directed mutational analy ...
Methanosarcina thermophila
J. Biol. Chem.
276
45059-45064
2001
-
-
1
-
11
-
-
28
-
3
-
1
-
2
-
-
-
-
-
-
9
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
11
-
-
-
-
28
-
3
-
1
-
-
-
-
-
-
9
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642197
Yu
Purification and characterizat ...
Desulfovibrio vulgaris, Desulfovibrio vulgaris Miyazaki F.
J. Biochem.
129
411-421
2001
-
-
-
-
-
-
1
1
-
1
3
2
-
4
-
-
1
-
-
-
2
1
2
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
3
2
-
-
-
1
-
-
2
1
2
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
727737
Buss
Urkinase: structure of acetate ...
Methanosarcina thermophila
J. Bacteriol.
183
680-686
2001
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642193
Ingram-Smith
The role of histidines in the ...
Methanosarcina thermophila
J. Biol. Chem.
275
33765-33770
2000
-
-
1
-
9
-
1
29
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
9
-
-
1
-
29
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642194
Singh-Wissmann
Identification of essential ar ...
Methanosarcina thermophila
Biochemistry
39
3671-3677
2000
-
-
1
-
7
-
1
19
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
1
-
19
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487537
Bock
Purification and characterizat ...
Thermotoga maritima
J. Bacteriol.
181
1861-1867
1999
-
-
-
-
-
1
-
4
-
6
2
1
-
7
1
-
1
-
-
-
-
1
7
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
4
-
6
2
1
-
1
-
1
-
-
-
1
7
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
642190
Lin
-
Purification and characterizat ...
Hungateiclostridium thermocellum
Tetrahedron
54
15915-15925
1998
-
1
-
-
-
-
-
1
-
2
2
1
-
1
-
-
1
-
-
-
1
-
1
1
1
1
1
-
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
2
1
-
-
-
1
-
-
1
-
1
1
1
1
1
-
1
1
1
-
-
-
-
-
-
-
642191
Singh-Wissmann
Identification of essential gl ...
Methanosarcina thermophila
J. Bacteriol.
180
1129-1134
1998
-
-
1
-
3
-
2
21
-
-
-
1
-
3
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
2
-
21
-
-
-
1
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642188
Buss
Crystallization of acetate kin ...
Methanosarcina thermophila
Protein Sci.
6
2659-2662
1997
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642189
Winzer
-
Acetate kinase from Clostridiu ...
Clostridium acetobutylicum
Microbiology
143
3279-3286
1997
-
-
1
-
-
-
2
4
-
4
3
1
-
1
-
-
1
-
-
-
1
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
4
-
4
3
1
-
-
-
1
-
-
1
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642187
Diez-Gonzalez
The acetate kinase of Clostrid ...
Clostridium acetobutylicum, Clostridium acetobutylicum P262
Arch. Microbiol.
166
418-420
1996
-
-
-
-
-
-
1
3
-
2
2
-
-
3
-
-
1
-
-
-
1
-
4
1
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
2
2
-
-
-
-
1
-
-
1
-
4
1
-
-
-
4
1
-
-
-
-
-
-
-
-
-
642184
Aceti
-
Purification and characterizat ...
Methanosarcina thermophila
J. Biol. Chem.
249
15444-15448
1988
-
-
-
-
-
2
-
2
1
4
2
1
-
1
1
-
1
-
-
-
1
1
8
1
-
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
2
1
4
2
1
-
1
-
1
-
-
1
1
8
1
-
1
1
-
1
1
-
1
-
-
-
-
-
-
642185
Kim
Inactivation of Acinetobacter ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
956
103-109
1988
-
-
-
-
-
1
2
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642186
Mannens
-
Purification and immobilizatio ...
Desulfovibrio vulgaris
Biotechnol. Lett.
10
563-568
1988
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727804
Aceti
Purification and characterizat ...
Methanosarcina thermophila, Methanosarcina thermophila TM-1
J. Biol. Chem.
263
15444-15448
1988
-
-
-
-
-
1
1
2
-
4
3
2
-
3
1
-
1
-
-
1
-
1
14
1
1
-
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
2
-
4
3
2
-
1
-
1
-
1
-
1
14
1
1
-
1
-
1
1
-
1
-
1
1
-
-
-
642182
Fox
Isolation and characterization ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
J. Biol. Chem.
261
13487-13497
1986
-
-
-
-
-
2
6
11
-
-
4
3
-
44
-
-
2
-
-
-
-
1
10
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
11
-
-
4
3
-
-
-
2
-
-
-
1
10
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
642183
Vigenschow
Purification and properties of ...
Rhodopseudomonas palustris
Biol. Chem. Hoppe-Seyler
367
951-956
1986
-
-
-
-
-
-
-
11
-
2
4
1
-
2
-
-
1
-
-
-
1
2
8
1
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
11
-
2
4
1
-
-
-
1
-
-
1
2
8
1
-
-
1
-
1
-
-
1
-
-
-
-
-
-
642181
Ichikawa
-
Purification and properties of ...
Propionibacterium freudenreichii
J. Ferment. Technol.
63
143-149
1985
-
-
-
-
-
-
-
6
-
6
2
1
-
1
-
-
1
-
-
-
1
-
5
1
1
-
1
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
6
2
1
-
-
-
1
-
-
1
-
5
1
1
-
1
-
2
1
1
-
-
-
-
-
-
-
642179
Harwood
Properties of acetate kinase i ...
Spirochaeta sp., Spirochaeta sp. MA-2
J. Bacteriol.
152
246-254
1982
-
-
-
-
-
-
-
6
-
6
2
2
-
2
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
6
2
2
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642180
Suzuki
-
Acetate kinase from Bacillus s ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus NCA 1503
Methods Enzymol.
90
179-185
1982
3
-
-
1
-
-
-
-
-
3
2
2
-
13
-
-
1
-
-
-
1
-
10
1
-
-
1
-
1
-
1
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
3
2
2
-
-
-
1
-
-
1
-
10
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
642178
Nishimura
-
Acetate kinase from Veilonella ...
Veillonella parvula, Veillonella parvula 221 / ATCC 17745
Methods Enzymol.
71
311-316
1981
1
-
-
-
-
-
-
-
-
3
2
2
-
2
-
-
1
-
-
-
1
1
10
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
2
2
-
-
-
1
-
-
1
1
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642177
Wong
Inactivation of Escherichia co ...
Escherichia coli
Biochim. Biophys. Acta
615
121-131
1980
-
-
-
-
-
1
3
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642174
Griffith
Acetate kinase from Veillonell ...
Veillonella parvula
J. Biol. Chem.
254
442-446
1979
1
-
-
-
-
-
5
-
-
-
2
1
-
2
-
-
1
-
-
-
1
-
1
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
-
-
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
642175
Griffith
Acetate kinase from Veillonell ...
Veillonella parvula
J. Biol. Chem.
254
6698-6702
1979
1
-
-
-
-
-
2
4
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
642176
Kahane
Purification and properties of ...
Acholeplasma laidlawii, Acholeplasma laidlawii oral
J. Bacteriol.
137
764-772
1979
-
-
-
-
-
1
6
6
-
1
5
2
-
3
-
-
1
-
-
-
1
1
11
1
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
6
-
1
5
2
-
-
-
1
-
-
1
1
11
1
-
-
-
5
-
-
-
-
-
-
-
-
-
-
642172
Nakajima
Purification and properties of ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus NCA 1503
J. Biochem.
84
193-203
1978
-
-
-
1
-
1
2
-
-
6
3
2
-
14
1
-
1
-
-
-
1
-
11
1
-
-
1
-
1
1
1
-
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
6
3
2
-
1
-
1
-
-
1
-
11
1
-
-
1
-
1
1
1
-
-
-
-
-
-
-
642173
Yoshimura
Purification and characterizat ...
Veillonella parvula
Arch. Biochem. Biophys.
189
424-432
1978
1
-
-
-
-
-
9
4
-
5
2
1
-
3
-
-
1
-
-
-
1
2
5
1
-
-
1
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
9
-
4
-
5
2
1
-
-
-
1
-
-
1
2
5
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
642171
Bowman
Acetate kinase from Veillonell ...
Veillonella parvula
J. Biol. Chem.
251
3117-3121
1976
1
-
-
-
-
-
-
10
-
-
2
1
-
2
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
10
-
-
2
1
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642168
Janson
The inhibition of acetate, pyr ...
Escherichia coli
J. Biol. Chem.
249
2567-2571
1974
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642169
Janson
The specificity of chromium nu ...
Escherichia coli
J. Biol. Chem.
249
2572-2574
1974
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642170
Schaupp
Purification and properties of ...
Moorella thermoacetica
Arch. Microbiol.
100
121-129
1974
-
-
-
-
-
-
-
1
-
-
1
1
-
2
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
642167
Anthony
Exchange reactions catalyzed b ...
Escherichia coli
J. Biol. Chem.
246
6129-6135
1971
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
-
-
1
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
1
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
642166
Rose
-
Acetate kinase ...
Escherichia coli, Lactobacillus delbrueckii, Streptococcus pyogenes
The Enzymes, 2nd. Ed. (Boyer, P. D. , Lardy, H. , Myrbaeck, K. , eds. )
6
115-118
1962
-
-
-
-
-
1
3
4
-
1
-
3
-
3
-
-
2
-
-
-
1
-
7
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
3
-
4
-
1
-
3
-
-
-
2
-
-
1
-
7
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-